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Updated: Jan 20, 2026

Injection of Microbubbles into Isolated Mouse Embryos: A Technique to Deliver Microbubble Contrast Agents into the Vasculature of Living Murine Embryos
Contrast Agent Microbubble Jetting during Initial Interaction with 200-kHz Focused Ultrasound
Sarah Cleve1, Claude Inserra2, Paul Prentice3
1Université Lyon, École Centrale de Lyon, INSA de Lyon, CNRS, LMFA UMR 5509, Écully, France.
Abstract:
The initial response of microbubbles flowing through a 500-μm polycarbonate capillary to a burst of 200-kHz focused ultrasound, at peak-negative pressure amplitudes from 0.7-1.5 MPa, was investigated with dual-perspective high-speed imaging. Directed jetting through the acoustic focus is demonstrated according to the pressure gradients acting across the cavitating microbubbles. At lower amplitudes, repeated microbubble-jetting is accompanied by sudden, intermittent translation. At higher amplitudes a rebound jet also forms, before disintegration into a cavitation cloud.
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