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New markers for Eubacterium lentum.

V D Bokkenheuser, J Winter, S M Finegold

    Applied and Environmental Microbiology
    |May 1, 1979
    PubMed
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    Many Eubacterium lentum strains and similar organisms possess steroid-metabolizing enzymes, specifically corticoid 21-dehydroxylase and 3 alpha-hydroxysteroid dehydrogenase. These enzymes are useful for classifying bacteria into steroid-active and steroid-inactive groups.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Enzymology

    Background:

    • Eubacterium lentum and phenotypically similar organisms are common gut bacteria.
    • Steroid metabolism by microorganisms is an area of interest for its potential applications.

    Purpose of the Study:

    • To investigate the presence and activity of steroid-metabolizing enzymes in Eubacterium lentum and related species.
    • To determine if these enzymes can be used as markers for bacterial classification.

    Main Methods:

    • Enzyme assays were performed on 37 strains of Eubacterium lentum and phenotypically similar organisms.
    • Steroid-metabolizing enzyme activities, including corticoid 21-dehydroxylase and 3 alpha-hydroxysteroid dehydrogenase, were measured.
    • Standard microbiological tests were used to characterize the strains, including nitrate reduction and growth in the presence of arginine.

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    Main Results:

    • 70% of the tested strains synthesized corticoid 21-dehydroxylase and/or 3 alpha-hydroxysteroid dehydrogenase.
    • The corticoid 3 alpha-hydroxysteroid dehydrogenase was found to be identical to the bile acid 3 alpha-hydroxysteroid dehydrogenase.
    • Steroid-metabolizing enzymes were found in both E. lentum and phenotypically similar organisms.
    • A clear distinction between "steroid-active" and "steroid-inactive" groups was established using these enzymes as markers.
    • Synthesis of steroid enzymes correlated positively with gas production from H2O2 and H2S formation.
    • A simple detection method for these enzymes identified them in 92% of the steroid-active group.

    Conclusions:

    • Eubacterium lentum and related bacteria possess significant steroid-metabolizing enzyme activity.
    • These enzymes serve as reliable markers for classifying bacteria into steroid-active and steroid-inactive groups.
    • The identified enzymes and detection methods offer potential for further research and applications in microbial steroid transformations.