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An Open-Source Normothermic Perfusion System Designed for Research Scientists11:23

An Open-Source Normothermic Perfusion System Designed for Research Scientists

Here, we present a protocol for an open-source, modifiable normothermic ex vivo perfusion system designed to overcome the high cost and limited flexibility of existing platforms, enabling more accessible, cost-efficient, and iterative use in pre-clinical biomedical...
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Deploying Community Scientists to Conduct Nondestructive Genetic Sampling of Rare Butterfly Populations07:17

Deploying Community Scientists to Conduct Nondestructive Genetic Sampling of Rare Butterfly Populations

Here, we present a straightforward protocol for noninvasive genetic sampling of butterfly populations based on the field collection of residual egg debris. It can be used to confirm species identity and quantify genetic variation. This protocol can be easily adapted to broader groups for community science...
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Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information09:37

Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information

Here, we present a protocol to access and analyze many human and model organism databases efficiently. This protocol demonstrates the use of MARRVEL to analyze candidate disease-causing variants identified from next-generation sequencing...
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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale08:49

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

This protocol describes a straightforward method to cultivate three-dimensional reconstituted human epidermis in a reproducible and robust manner. Additionally, it characterizes the structure-function relationship of the epidermal barrier model. The biological responses of the reconstituted human epidermis upon proinflammatory stimuli are also...
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Biodiversity and Human Values01:24

Biodiversity and Human Values

Human civilization relies on biodiversity in many ways. Sudden changes in species biodiversity result in environmental changes that can modify weather patterns and therefore human civilizations.
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Gene Expression Analyses in Human Follicles09:09

Gene Expression Analyses in Human Follicles

Here, we describe a protocol outlining how to isolate human ovarian follicles from frozen-thawed cortical tissue to perform gene expression analyses.
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