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Related Concept Videos

Lysosomes01:31

Lysosomes

25.4K
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
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Lysosomal Hydrolases01:22

Lysosomal Hydrolases

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Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
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Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome07:56

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

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Mitophagy is the primary mechanism of mitochondrial quality control. However, the evaluation of mitophagy in vivo is hindered by the lack of reliable quantitative assays. Presented here is a protocol for the observation of mitophagy in living cells using a cell-permeant green-fluorescent mitochondria dye and a red-fluorescent lysosome dye.
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Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

8.8K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
8.8K
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

2.9K
A neuronal lysosome proximity labeling proteomics protocol is described here to characterize the dynamic lysosomal microenvironment in human induced pluripotent stem cell-derived neurons. Lysosomal membrane proteins and proteins that interact with lysosomes (stably or transiently) can be accurately quantified in this method with excellent intracellular spatial resolution in live human...
2.9K
Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes10:25

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

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This protocol describes a mechanism for using correlative light and electron microscopy to visualize the interaction of mitochondria and lysosomes labeled with mEosEM and APEX2,...
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Related Experiment Video

Updated: Jan 20, 2026

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
07:56

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

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Lysosomes as a therapeutic target.

Srinivasa Reddy Bonam1,2, Fengjuan Wang1,2, Sylviane Muller3,4,5,6

  • 1CNRS-University of Strasbourg, Biotechnology and Cell Signalling, Illkirch, France.

Nature Reviews. Drug Discovery
|September 4, 2019
PubMed
Summary
This summary is machine-generated.

Lysosomes are crucial for cellular health. Targeting lysosomal dysfunction offers new therapeutic avenues for autoimmune and neurodegenerative diseases like Alzheimer's and Parkinson's.

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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

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Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
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Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

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Related Experiment Videos

Last Updated: Jan 20, 2026

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
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Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

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Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
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Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

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Area of Science:

  • Cell Biology
  • Molecular Medicine
  • Neuroscience

Background:

  • Lysosomes are vital organelles involved in cellular waste degradation, signaling, and metabolism.
  • Lysosomal dysfunction is linked to storage disorders and increasingly implicated in common diseases.
  • Specific focus on autoimmune (lupus, RA, MS) and neurodegenerative (Alzheimer's, Parkinson's) diseases.

Purpose of the Study:

  • To critically analyze therapeutic targeting of lysosomal proteins and processes.
  • To review progress and opportunities for treating diseases linked to lysosomal dysfunction.
  • To explore the potential of small molecules and peptide drugs for lysosomal therapies.

Main Methods:

  • Literature review and critical analysis of existing research.
  • Focus on studies investigating lysosomal dysfunction in specific autoimmune and neurodegenerative conditions.
  • Examination of therapeutic strategies targeting lysosomal proteins and pathways.

Main Results:

  • Lysosomal dysfunction is a significant factor in autoimmune and neurodegenerative diseases.
  • Enzyme replacement therapy is effective for lysosomal storage disorders.
  • Emerging evidence supports targeting lysosomal pathways for broader therapeutic benefit.

Conclusions:

  • Targeting lysosomal dysfunction presents promising therapeutic opportunities.
  • Small molecules and peptide drugs are key modalities for future lysosomal therapies.
  • Further research is needed to fully exploit lysosomal pathways for treating complex diseases.