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Preparation of a High-quality Primary Cell Culture from Fish Pituitaries07:36

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

15.9K
Here we describe a protocol to prepare and maintain primary pituitary cell cultures from medaka (Oryzias latipes). The optimized conditions in this protocol take important parameters such as temperature, osmolality, and pH into consideration by mimicking the physiological conditions of the fish, thereby enabling physiologically more meaningful...
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Hypothalamic-Pituitary Axis01:37

Hypothalamic-Pituitary Axis

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The response to stress—be it physical or psychological, acute or chronic—involves activation of the Hypothalamic-Pituitary-Adrenal (HPA) axis. The HPA axis is part of the neuroendocrine system because it involves both neuronal and hormonal communication. Its function is to regulate homeostatic systems—metabolic, cardiovascular, and immune—providing the necessary means to respond to a stressor.
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Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology09:48

Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

4.6K
The pituitary gland is the key regulator of the body's endocrine system. This article describes the development of organoids from the mouse pituitary as a novel 3D in vitro model to study the gland's stem cell population of which the biology and function remain poorly...
4.6K
Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish07:14

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish

8.2K
The article describes an optimized protocol for making viable brain-pituitary tissue slices, using the teleost fish medaka (Oryzias latipes), followed by electrophysiological recordings of pituitary cells using the patch-clamp technique with the perforated patch...
8.2K
Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells08:31

Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells

10.4K
Three-dimensional culture in alginate beads, due to its simplicity and reproducibility, was chosen to maintain the pituitary adenoma cells. The procedures included an initial enzymatic digestion and mechanical dissociation of the tumor tissue, and the subsequent cell suspension was encapsulated in alginate...
10.4K
Hormones of the Pituitary Gland01:27

Hormones of the Pituitary Gland

9.6K
The small, pea-sized pituitary gland is located at the base of the brain. It is crucial in regulating various bodily functions, from growth to reproduction. The gland is divided into the anterior lobe and the posterior lobe. The secretory cell clusters in the pars distalis of the anterior pituitary lobe are controlled by hypothalamic regulators and synthesize six primary hormones.
The most abundantly secreted hormone from the anterior lobe is the growth hormone, which controls overall growth by...
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Related Experiment Video

Updated: Jan 20, 2026

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries
07:36

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

Published on: August 28, 2018

15.9K

Primary Pituitary Tubercular Abscess: A Case Report.

Yam Bahadur Roka1, Narayani Roka2, Sagar Raj Pandey3

  • 1Department of Neurosurgery, Neuro Cardio and MultiSpeciality Hospital, Biratnagar, Nepal.

JNMA; Journal of the Nepal Medical Association
|September 4, 2019
PubMed
Summary
This summary is machine-generated.

Primary pituitary tubercular abscess is a rare condition presenting with various neurological symptoms. Successful management involves surgery followed by anti-tubercular therapy.

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Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology
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Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
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Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish

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Related Experiment Videos

Last Updated: Jan 20, 2026

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries
07:36

Preparation of a High-quality Primary Cell Culture from Fish Pituitaries

Published on: August 28, 2018

15.9K
Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology
09:48

Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology

Published on: February 25, 2022

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Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish
07:14

Healthy Brain-pituitary Slices for Electrophysiological Investigations of Pituitary Cells in Teleost Fish

Published on: August 16, 2018

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Area of Science:

  • Endocrinology
  • Neurology
  • Infectious Diseases

Background:

  • Primary pituitary tuberculosis is an exceptionally rare condition.
  • It can manifest with diverse clinical symptoms including visual impairment, headaches, seizures, hormonal imbalances, and cranial nerve palsies.

Observation:

  • Magnetic Resonance Imaging (MRI) is crucial for diagnosis, revealing a cystic-solid mass in the sellar/suprasellar region.
  • The mass typically appears isointense on T1 and T2-weighted images, with heterogeneous areas and ring enhancement post-contrast.

Findings:

  • Surgical intervention, via trans-sphenoidal/trans-nasal or transcranial approaches, is the primary treatment.
  • Post-operative anti-tubercular therapy is essential for successful management.

Implications:

  • This case highlights the importance of considering tuberculosis in the differential diagnosis of sellar masses.
  • Effective management combines surgical decompression with appropriate anti-mycobacterial treatment for favorable outcomes.