Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration08:55

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

2.4K
This protocol describes the assembly of a layer-by-layer Janus base nano-matrix (JBNm) scaffold by adding Janus base nanotubes (JBNts), matrilin-3, and Transforming Growth Factor Beta-1 (TGF-β1) sequentially. The JBNm was fabricated and characterized; additionally, it displayed excellent bioactivity, encouraging cell functions such as adhesion, proliferation, and differentiation.
2.4K
3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration09:46

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

10.2K
Chondrogenesis from stem cells requires fine tuning the culture conditions. Here, we present a magnetic approach for condensing cells, an essential step to initiate chondrogenesis. In addition, we show that dynamic maturation in a bioreactor applies mechanical stimulation to the cellular constructs and enhances cartilaginous extracellular matrix...
10.2K
Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration14:49

Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration

42.4K
Here, we present a protocol to produce an adipose tissue-derived stromal vascular fraction and its application to improve knee functions by regenerating cartilage-like tissue in human patients with osteoarthritis.
42.4K
Atomic Force Microscopy Measurements of Cartilage in Intact and Regenerating Axolotl Limbs09:19

Atomic Force Microscopy Measurements of Cartilage in Intact and Regenerating Axolotl Limbs

1.3K
In this protocol, we show how to prepare axolotl tissue for atomic force microscopy (AFM) and perform indentation measurements in intact and regenerating limb...
1.3K
A Cross-Disciplinary and Multi-Modal Experimental Design for Studying Near-Real-Time Authentic Examination Experiences08:33

A Cross-Disciplinary and Multi-Modal Experimental Design for Studying Near-Real-Time Authentic Examination Experiences

7.4K
An experimental design was developed to investigate the real-time influences of an examination experience to assess the emotional realities students experience in higher education settings and tasks. This design is the result of a cross-disciplinary (e.g., educational psychology, biology, physiology, engineering) and multi-modal (e.g., salivary markers, surveys, electrodermal sensor)...
7.4K
Studying the Regeneration of Functional Connections between Spinal Cord Slices Using a Multi-Electrode Array03:52

Studying the Regeneration of Functional Connections between Spinal Cord Slices Using a Multi-Electrode Array

387
The video demonstrates the functional regeneration of propriospinal connections using a multi-electrode array (MEA)-based assay. The spinal cord slices are placed on the MEA and are allowed to fuse. The fused slices are mechanically separated by creating a lesion, inducing propriospinal neurons to grow over the lesion and connect the slices. The electrical activity of the slices is assessed to identify the regeneration of functional...
387

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

YAP1 Knockdown Reduces IL-1β-Induced Human Chondrocyte Inflammation and Promotes Human MSC Chondrogenesis.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Biomimetic 3D-printed gyroid scaffolds with versatile bioactive coatings for complex craniomaxillofacial bone regeneration.

Biomedical materials (Bristol, England)·2026
Same author

Antibodies blocking PlGF or VEGF interactions with the NRP1 receptor mediate anti-proliferative effects.

bioRxiv : the preprint server for biology·2025
Same author

Transient Early Mechanical Loading Induces Hypertrophic Chondrocyte Differentiation of Human Mesenchymal Stromal Cells.

Cells·2025
Same author

What's New in Musculoskeletal Basic Science.

The Journal of bone and joint surgery. American volume·2025
Same author

Donor-dependent regulation of type II and X collagen deposition by early modulation of miR-335-5p and miR-1246 during chondrogenic commitment.

Stem cell research & therapy·2025

Related Experiment Video

Updated: Jan 20, 2026

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration
08:55

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Published on: July 6, 2022

2.4K

Multi-Disciplinary Approaches for Cell-Based Cartilage Regeneration.

Brian Johnstone1, Martin J Stoddart2, Gun-Il Im3

  • 1Department of Orthopaedics and Rehabilitation, Oregon Health & Science University, Portland, Oregon.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|September 4, 2019
PubMed
Summary

Articular cartilage regeneration remains a challenge. A multidisciplinary approach combining biological, genetic, and mechanical methods is key for advancing cell-based cartilage repair strategies.

Keywords:
biomechanicscartilagegene therapyprogenitors and stem cells

More Related Videos

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
09:46

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

Published on: April 27, 2017

10.2K
Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration
14:49

Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration

Published on: September 29, 2018

42.4K

Related Experiment Videos

Last Updated: Jan 20, 2026

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration
08:55

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Published on: July 6, 2022

2.4K
3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
09:46

3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration

Published on: April 27, 2017

10.2K
Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration
14:49

Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration

Published on: September 29, 2018

42.4K

Area of Science:

  • Orthopaedic Research
  • Biomedical Engineering
  • Regenerative Medicine

Background:

  • Articular cartilage has limited self-repair capacity in adults.
  • Significant research efforts focus on cartilage regeneration strategies.
  • Current understanding points to a multidisciplinary approach for effective regeneration.

Purpose of the Study:

  • To review key approaches for cell-based cartilage regeneration.
  • To summarize findings from a workshop on cartilage regeneration.
  • To highlight advancements in biological, genetic, and mechanical stimulation techniques.

Main Methods:

  • Review of a workshop sponsored by International Combined Orthopaedic Societies.
  • Discussion of cellular basis of chondrogenesis.
  • Exploration of gene-enhanced cartilage regeneration.
  • Analysis of physical modulation for stem cell differentiation.

Main Results:

  • Three primary cell-based cartilage regeneration strategies were presented.
  • Challenges in achieving hyaline articular cartilage regeneration persist.
  • The integration of diverse biomedical engineering fields is crucial.

Conclusions:

  • Successful articular cartilage regeneration requires a multifaceted strategy.
  • Advancements in gene therapy and physical modulation show promise.
  • Continued multidisciplinary research is essential for future breakthroughs in cartilage repair.