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Related Concept Videos

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations06:19

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The present protocol describes a convenient approach to integrating optical trapping and surface-enhanced Raman spectroscopy (SERS) to manipulate plasmonic nanoparticles for sensitive molecular detection. Without aggregating agents, the trapping laser assembles plasmonic nanoparticles to enhance the SERS signals of target analytes for in situ spectroscopic...
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We describe the fabrication and characterization of nano-biological systems interfacing nanostructured substrates with immobilized proteins and aptamers. The relevant experimental steps involving lithographic fabrication of nanostructured substrates, bio-functionalization, and surface-enhanced Raman spectroscopy (SERS) characterization, are reported. SERS detection of surface-immobilized proteins, and probing of protein-ligand and aptamer-ligand binding is demonstrated.
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Source: Laboratory of Dr. Ryoichi Ishihara — Delft University of Technology
Raman spectroscopy is a technique for analyzing vibrational and other low frequency modes in a system. In chemistry it is used to identify molecules by their Raman fingerprint. In solid-state physics it is used to characterize materials, and more specifically to investigate their crystal structure or crystallinity. Compared to other techniques for investigating the crystal structure (e.g. transmission electron...
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Updated: Jan 20, 2026

Optical Trapping of Plasmonic Nanoparticles for In Situ Surface-Enhanced Raman Spectroscopy Characterizations
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Extending Surface-Enhanced Raman Spectroscopy to Liquids Using Shell-Isolated Plasmonic Superstructures.

Caterina S Wondergem1, Thomas P van Swieten1, Robin G Geitenbeek1

  • 1Inorganic Chemistry and Catalysis Group, Debye Institute for Nanomaterials Science, Utrecht University, Universiteitsweg 99, 3584, CG, Utrecht, The Netherlands.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|September 4, 2019
PubMed
Summary

Shell-Isolated Plasmonic Superstructures (SHIPS) were developed for enhanced Raman spectroscopy. These structures improve sensitivity for liquid-phase applications, achieving a detection limit of 10-12 M Rhodamine.

Keywords:
Raman spectroscopySERSheterogeneous catalysisplasmonic nanoparticlesreaction monitoring

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Area of Science:

  • Nanotechnology
  • Materials Science
  • Spectroscopy

Background:

  • Plasmonic superstructures (PS) are crucial for enhancing spectroscopic signals.
  • Liquid-phase applications require robust nanoparticle systems resistant to aggregation and leaching.
  • Shell-Isolated Nanoparticle-Enhanced Raman Spectroscopy (SHINERS) offers high sensitivity but needs stable platforms.

Purpose of the Study:

  • To develop and characterize novel plasmonic superstructures for liquid-phase SHINERS.
  • To optimize the synthesis of gold nanoparticles on functionalized silica spheres.
  • To evaluate the performance of the resulting Shell-Isolated Plasmonic Superstructures (SHIPS) for sensitive analyte detection.

Main Methods:

  • Synthesis of silica spheres functionalized with poly(ethylene-imine) (PEI).
  • Adsorption of gold nanoparticles (Au NPs) onto functionalized silica spheres.
  • Coating of the plasmonic superstructures with an ultrathin SiO2 layer to create SHIPS.
  • Characterization using Transmission Electron Microscopy (TEM), UV/Vis spectroscopy, Dynamic Light Scattering (DLS), and Zeta potential measurements.
  • Evaluation of SHIPS performance using SHINERS with Rhodamine as a model analyte.

Main Results:

  • PEI-functionalized silica spheres demonstrated the highest adsorption density of Au NPs.
  • Decreasing the Au NP/SiO2 sphere size ratio increased nanoparticle adsorption.
  • Successful formation of SHIPS with an ultrathin SiO2 shell confirmed by characterization techniques.
  • SHIPS achieved a detection limit of approximately 10-12 M Rhodamine, significantly enhancing sensitivity compared to uncoated structures (10-15 M).

Conclusions:

  • Poly(ethylene-imine) functionalization and controlled size ratios are key for creating dense Au NP coverage on SiO2 spheres.
  • The SiO2 coating effectively isolates plasmonic nanoparticles, enabling stable and sensitive SHINERS in liquid phase.
  • The developed SHIPS platform demonstrates excellent potential for sensitive detection in liquid-phase applications, including catalysis.