Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies08:04

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

4.0K
This paper describes a semiautomated, microscaled ChIP-Seq protocol of DNA regions associated with a specific histone modification (H3K27ac) using a ChIP liquid-handler platform, followed by library preparation using tagmentation. The procedure includes control assessment for quality and quantity purposes and can be adopted to other histone modifications or transcription...
4.0K
Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq10:23

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

12.9K
The question of how chromatin regulators and chromatin states affect the genome in vivo is key to our understanding of how early cell fate decisions are made in the developing embryo. ChIP-Seq—the most popular approach to investigate chromatin features at a global level—is outlined here for Xenopus...
12.9K
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

10.2K
Here, we describe methods to perform ChIP-Seq and CARIP-Seq, including library preparation for next-generation sequencing, to generate global epigenomic and chromatin-associated RNA maps in ES...
10.2K
On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature07:42

On-Chip Crystallization and Large-Scale Serial Diffraction at Room Temperature

2.3K
This contribution describes how to set up protein crystallization on crystal-on-crystal devices and how to perform automated serial data collection at room temperature using the on-chip crystallization...
2.3K
RNA-Seq09:54

RNA-Seq

73.5K
Among different methods to evaluate gene expression, the high-throughput sequencing of RNA, or RNA-seq. is particularly attractive, as it can be performed and analyzed without relying on prior available genomic information. During RNA-seq, RNA isolated from samples of interest is used to generate a DNA library, which is then amplified and sequenced. Ultimately, RNA-seq can determine which genes are expressed, the levels of their expression, and the presence of any previously unknown...
73.5K
Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells08:34

Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells

19.1K
Methods for mapping in vivo protein-DNA interactions are becoming crucial for every aspect of genomic research but they are laborious, costly, and time consuming. Here a commercially available robotic liquid handling system that automates chromatin immunoprecipitation for mapping in vivo protein-DNA interactions with limited amounts of cells is...
19.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Longitudinal analysis of the hand microbiome in response to chlorine-based antiseptic use during a military field exercise.

Microbiology spectrum·2026
Same author

Topological stress regulates replication fork dynamics in unperturbed S phase.

Nature communications·2026
Same author

CDK12/CDK13 inhibition disrupts transcriptional elongation and replication fork progression in glioblastoma.

EMBO molecular medicine·2026
Same author

Findings on Magnetic Resonance Imaging in Neuroborreliosis-A Nationwide Cohort Study.

European journal of neurology·2026
Same author

Outcome of Pediatric Large Vessel Occlusion Stroke in Denmark.

Stroke (Hoboken, N.J.)·2026
Same author

Author Correction: Loss of the histone methyltransferase EZH2 induces resistance to multiple drugs in acute myeloid leukemia.

Nature medicine·2026

Related Experiment Video

Updated: Jan 20, 2026

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies
08:04

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

Published on: August 13, 2020

4.0K

Going low to reach high: Small-scale ChIP-seq maps new terrain.

Madeleine Fosslie1, Adeel Manaf1, Mads Lerdrup2,3

  • 1Department of Microbiology, Oslo University Hospital, Oslo, Norway.

Wiley Interdisciplinary Reviews. Systems Biology and Medicine
|September 4, 2019
PubMed
Summary

Small-scale Chromatin Immunoprecipitation sequencing (ChIP-seq) now allows genome-wide chromatin profiling from limited cell numbers. This review covers methods, data analysis, and applications for studying chromatin dynamics in rare cell populations.

Keywords:
H3K4me3chromatin immunoprecipitationhistone modificationlow cell number ChIP-seqoocyte

More Related Videos

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq
10:23

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Published on: February 26, 2015

12.9K
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

10.2K

Related Experiment Videos

Last Updated: Jan 20, 2026

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies
08:04

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

Published on: August 13, 2020

4.0K
Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq
10:23

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Published on: February 26, 2015

12.9K
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells
11:13

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Published on: May 25, 2018

10.2K

Area of Science:

  • Epigenetics and Genomics
  • Developmental Biology
  • Cell Biology

Background:

  • Chromatin Immunoprecipitation (ChIP) and ChIP sequencing (ChIP-seq) are crucial for mapping genomic regulatory elements.
  • Traditional ChIP-seq requires substantial cell input, limiting its application to rare or primary cell types.
  • Working with pure cell populations is advantageous for robust biological conclusions in chromatin studies.

Purpose of the Study:

  • To review scalable ChIP protocols for limited cell inputs.
  • To discuss considerations for small-scale ChIP-seq library preparation and data analysis.
  • To highlight the potential of small-scale ChIP-seq for understanding chromatin dynamics in developmental processes.

Main Methods:

  • Review of scaled-down ChIP protocols adaptable for hundreds of cells.
  • Guidance on preparing and analyzing small-scale ChIP-seq data.
  • Exploration of ChIP-seq applications using limited cell material.

Main Results:

  • ChIP protocols can be effectively scaled down to accommodate very few cells.
  • Specific considerations are necessary for successful small-scale ChIP-seq library preparation and data interpretation.
  • Small-scale ChIP-seq enables chromatin profiling in previously inaccessible cell types.

Conclusions:

  • Small-scale ChIP-seq overcomes input material limitations, expanding the scope of chromatin studies.
  • This technique is valuable for investigating chromatin dynamics during oocyte maturation and preimplantation development.
  • Small-scale ChIP-seq facilitates robust biological conclusions from limited cell populations, advancing developmental biology and cell fate research.