Related Experiment Video

Updated: Jan 20, 2026

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
10:36

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

Published on: January 9, 2018

7.5K

Natural killer cell-based immunotherapy with CTLA4Ig-primed donor lymphocytes following haploidentical

Sarita Rani Jaiswal1,2, Suparno Chakrabarti1,2

  • 1Cellular Therapy & Immunology, Manashi Chakrabarti Foundation, Kolkata.

Immunotherapy
|September 4, 2019
PubMed

NK cell-based immunotherapy is one of the more exciting propositions in the field of cellular therapy for hematological malignancies. Current protocols are largely based on expanded and activated NK cells which are used both with and without allogeneic transplantation. Based on our recent findings, we discuss the concept of CTLA4Ig-primed donor lymphocyte infusions following haploidentical transplantation as an effective tool to garner NK cell-mediated antitumor effect with abrogation of T cell-mediated alloreactivity. This approach might widen the possibility of immunotherapy following haploidentical transplantation without increase in graft-versus-host disease. Further studies would be needed to establish the veracity of this concept with better understanding of the antitumor effect via this pathway. Future studies would decide if CTLA4Ig might be used to augment NK-cell activation in vitro as well.

Keywords:
CD28CD80-86CTLA4IgGVHDNK cellTregshaploidenticalimmunotherapyleukemialymphomamyeloma

More Related Videos

Analysis of Human Natural Killer Cell Metabolism09:03

Analysis of Human Natural Killer Cell Metabolism

Published on: June 22, 2020

7.4K
Natural Killer Cell Isolation from Liver Tissue for Selective Expansion04:17

Natural Killer Cell Isolation from Liver Tissue for Selective Expansion

805

Related Concept Videos

Natural Killer Cell Isolation from Liver Tissue for Selective Expansion04:17

Natural Killer Cell Isolation from Liver Tissue for Selective Expansion

This video demonstrates the isolation of lymphocytes, including natural killer cells or NK cells, from human liver tissue for selective expansion. Enzymatic and mechanical digestion, followed by density gradient separation, promotes lymphocyte isolation, including NK cells. The collected lymphocytes can be further used for the selective expansion of NK...
805
A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro10:36

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

Here, we present a protocol to mass-produce gene-silencing murine NK cells by using a feeder-free differentiation system for mechanistic study in vitro and in...
7.5K
Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells03:14

Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells

This video demonstrates a technique for the expansion of natural killer (NK) cells directly from peripheral blood mononuclear cells (PBMCs) using a feeder cell line. PBMCs are co-cultured with genetically modified lymphoblastoid feeder cells expressing membrane-bound interleukin-21 (mIL-21), and the culture is supplemented with the cytokines interleukin-2 (IL-2) and interleukin-15 (IL-15). Together, this cocktail of ligands leads to the prolonged expansion of NK cells with high...
1.0K
Analysis of Human Natural Killer Cell Metabolism09:03

Analysis of Human Natural Killer Cell Metabolism

In this paper, we describe a method to measure glycolysis and mitochondrial respiration in primary human Natural Killer (NK) cells isolated from peripheral blood, at rest or following IL15-induced activation. The protocol described could be easily extended to primary human NK cells activated by other cytokines or soluble...
7.4K
Isolating Invariant Natural Killer T Cells from a Mouse Model03:35

Isolating Invariant Natural Killer T Cells from a Mouse Model

This video illustrates a method for isolating invariant natural killer T cells, also known as iNKT cells. The procedure begins with injecting a synthetic glycolipid into a mouse to stimulate the activation and proliferation of iNKT cells in the spleen. Subsequently, single-cell suspensions are prepared from the spleen and incubated with iNKT-cell-recognizing complexes labeled with fluorophores, followed by magnetic bead-based separation, to isolate the iNKT...
647
Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line11:02

Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line

Here, we present a method to expand peripheral blood natural killer (PBNK), NK cells from liver tissues, and chimeric antigen receptor (CAR)-NK cells derived from peripheral blood mononuclear cells (PBMCs) or cord blood (CB). This protocol demonstrates the expansion of NK and CAR-NK cells using 221-mIL-21 feeder cells in addition to the optimized purity of expanded NK...
6.3K