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Micro-Colony Forming Unit Assay for Efficacy Evaluation of Vaccines Against Tuberculosis

The determination of colony-forming units (CFU) is the gold-standard technique for quantifying bacteria, including Mycobacterium tuberculosis which can take weeks to form visible colonies. Here we describe a micro-CFU for CFU determination with increased time efficiency, reduced lab space and reagent cost, and scalability to medium and high throughput...
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Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice08:52

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

Here we present an elegant protocol for in vivo evaluation of vaccine effectiveness and host immune responses. This protocol can be adapted for vaccine models that study viral, bacterial, or parasitic...
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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Here we present two protocols, one for measuring the specific growth rate and the other for the cell-binding ability of rotavirus using the plaque assay and RT-qPCR. These protocols are available for confirming the differences in phenotypes between rotavirus...
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Vaccinations01:51

Vaccinations

Overview
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Generating Recombinant Live-Attenuated Influenza Viruses for Vaccines02:58

Generating Recombinant Live-Attenuated Influenza Viruses for Vaccines

The video showcases a method to create cold-adapted recombinant influenza viruses. It involves transfecting human cells with bi-directional plasmids, followed by incubation at lower temperature incubation to yield viruses suitable for vaccine production, characterized by reduced replication in warmer...
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Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Generation of recombinant rotaviruses from plasmid DNA provides an essential tool for the study of rotavirus replication and pathogenesis, and the development of rotavirus expression vectors and vaccines. Herein, we describe a simplified reverse genetics approach for generating recombinant rotaviruses, including strains expressing fluorescent reporter...
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