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Updated: Jan 20, 2026

Cooperative Allosteric Transitions: Concerted & Sequential Model
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Crosslinking Allosteric Sites on the Nucleosome.

Lucinda K Batchelor1, Louis De Falco2, Thibaud von Erlach1

  • 1Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

Angewandte Chemie (International Ed. in English)
|September 4, 2019
PubMed
Summary

This study introduces a novel hetero-bimetallic compound that targets histone proteins in chromatin. This compound acts as a site-selective cross-linking agent, enhancing nucleosome stability for potential therapeutic applications.

Keywords:
allosterybioinorganic chemistrymacromolecular crystallographymolecular dynamics simulationsnucleosome structure

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Area of Science:

  • Biochemistry
  • Medicinal Chemistry
  • Structural Biology

Background:

  • Targeting histone proteins in chromatin is a promising therapeutic strategy.
  • Allosteric effects within the nucleosome can enhance therapeutic targeting.
  • Previous studies identified allosteric effects for ruthenium (RuII) and gold (AuI) compounds.

Purpose of the Study:

  • To characterize a novel hetero-bimetallic compound designed using nucleosomal allostery.
  • To investigate the compound's mechanism of action and its effect on nucleosome structure and stability.

Main Methods:

  • Design of a hetero-bimetallic compound incorporating RuII and AuI moieties.
  • Characterization of the compound's binding interactions with histone proteins (H2A, H3).
  • Assessment of nucleosome structural changes and stability upon compound application.

Main Results:

  • The RuII moiety binds to H2A glutamate residues, inducing allosteric changes.
  • These changes facilitate AuI binding to H3 histidine residues, creating distant cross-linking sites (>35 Å).
  • The compound enhances nucleosome stability by tethering histone dimers and tetramers.

Conclusions:

  • The novel hetero-bimetallic compound functions as a site-selective nucleosome cross-linking agent.
  • This compound leverages nucleosomal allostery for targeted therapeutic approaches.
  • The findings highlight the potential of allosteric modulation for stabilizing chromatin structures.