Related Experiment Video
Updated: Jan 20, 2026

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis
Published on: March 11, 2017
Sulpiride gastro-retentive floating microsponges; analytical study, in vitro optimization and in vivo
Mahmoud A Younis1, Marwa R El-Zahry2, Mahmoud A Tallat3
1Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
Abstract:
Sulpiride (SUL), anti-dopaminergic drug, has a specific site for absorption located in the upper portion of the gastrointestinal tract hence, its oral delivery represents a challenge regarding SUL absorption and bioavailability. So, a gastro-retentive oral platform of SUL was developed to increase its gastric residence time, release SUL at a controlled rate in the stomach and consequently, enable it to reach its specific absorption site. Floating microsponges were prepared via quasi-emulsion solvent diffusion method and characterised for their physico-chemical properties. In addition, Taguchi design of experiment was utilised to optimise some independent variables affecting microsponges performance. The optimised SUL microsponges showed a yield of 79.82 ± 2.37%, an encapsulation efficiency of 89.11 ± 2.28% and in vitro time for floatation of 8.0 h. Additionally, pharmacokinetics were investigated in rabbits and compared with the commercial SUL formulation, Dogmatil® capsules. Optimised SUL microsponges showed a significantly (p < .05) higher Cmax, AUC and 2-fold increase in oral bioavailability compared with the commercial product. Moreover, the optimised SUL microsponges remained present in the stomach up to 8.0 h post administration when viewed via X-ray radiographs in rabbits. It could be concluded that the floating microsponges could be useful as an oral platform to enhance the sulpiride absorption and bioavailability.
Related Concept Videos
08:58Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis
08:16In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
Sample Preparation for Analytical Characterization
Sample preparation is the way in which a sample is treated to prepare for analysis. Careful sample preparation is critical in analytical chemistry to accurately generate either a standard or unknown sample for a chemical measurement. Errors in analytical chemistry methods are categorized as random or systematic. Random errors are errors due to change and are often due to noise in instrument. Systematic errors are due to...
Stability of Floating Vessels
The objective of this experiment is to demonstrate the phenomenon of stability of floating vessels - the ability to self-right when rolled over to the side by some external force. Careful design of hull shapes and internal mass distribution enables seagoing vessels to be stable with low drafts (submerged depth of hull), improving vessel...
05:14Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo
17:14In vivo and in vitro Studies of Adaptor-clathrin Interaction
