Segmenting Cellular Retinal Images by Optimizing Super-pixels, Multi-level Modularity, and Cell Boundary

Related Concept Videos

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree optical tomography is a three-dimensional microscopy technique that offers a spatial resolution of <1 μm × <1 μm × <3 μm in x, y and z dimensions, respectively, over a large imaging-volume of 15-100 mm3, which can be particularly useful for integration with lab-on-a-chip...
11.9K
Volume Segmentation and Analysis of Biological Materials Using SuRVoS (Super-region Volume Segmentation) Workbench11:38

Volume Segmentation and Analysis of Biological Materials Using SuRVoS (Super-region Volume Segmentation) Workbench

Segmentation of three-dimensional data from many imaging techniques is a major bottleneck in analysis of complex biological systems. Here, we describe the use of SuRVoS Workbench to semi-automatically segment volumetric data at various length-scales using example datasets from cryo-electron tomography, cryo soft X-ray tomography, and phase contrast X-ray tomography...
10.2K
Ground State Depletion Super-resolution Imaging in Mammalian Cells07:55

Ground State Depletion Super-resolution Imaging in Mammalian Cells

This article outlines a protocol for the detection of one or more plasma and/or intracellular membrane proteins using Ground State Depletion (GSD) super-resolution microscopy in mammalian cells. Here, we discuss the benefits and considerations of using such approaches for the visualization and quantification of cellular...
7.5K
Super-Resolution Live Cell Imaging of Subcellular Structures06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Presented here is a protocol for super-resolution live-cell imaging in intact tissue. We have standardized the conditions for imaging a highly sensitive adult stem cell population in its native tissue environment. This technique involves balancing temporal and spatial resolution to allow for the direct observation of biological phenomena in live...
5.2K
Test Samples for Optimizing STORM Super-Resolution Microscopy16:52

Test Samples for Optimizing STORM Super-Resolution Microscopy

We describe the preparation of three test samples and how they can be used to optimize and assess the performance of STORM microscopes. Using these examples we show how to acquire raw data and then process it to acquire super-resolution images in cells of approximately 30-50 nm...
31.6K
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization06:33

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

This protocol explains how to prepare and mount bacterial samples for live three-dimensional imaging and how to reconstruct the three-dimensional shape of E. coli from those...
10.7K