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Iris Fixation via External Pentagram Suturing05:22

Iris Fixation via External Pentagram Suturing

The present protocol describes how a 10-0 polypropylene suture crosses the anterior surface of the iris, forming a pentagram to prevent both iris and pupil from moving toward the cornea. This can be combined with subsequent keratoplasty to cure bullous keratopathy with extensive anterior synechia of the...
1.8K
A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice09:03

A Reversible Silicon Oil-Induced Ocular Hypertension Model in Mice

Here, we present a protocol to induce ocular hypertension and glaucomatous neurodegeneration in mouse eyes by intracameral injection of silicone oil and the procedure for silicone oil removal from the anterior chamber to return elevated intraocular pressure to...
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Puncture-Induced Iris Neovascularization as a Mouse Model of Rubeosis Iridis06:57

Puncture-Induced Iris Neovascularization as a Mouse Model of Rubeosis Iridis

Iris neovascularization, a common complication of ischemic retinal disease, may lead to sight-threatening neovascular glaucoma. Here, we describe a murine protocol for inducing experimental iris neovascularization that may be used for noninvasive evaluation of angiogenesis-modulating...
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Improved Polydimethylsiloxane (PDMS) Double Casting via Silicone Oil Treatment for Densely Packed Microstructure Replication07:01

Improved Polydimethylsiloxane (PDMS) Double Casting via Silicone Oil Treatment for Densely Packed Microstructure Replication

This protocol presents an improved demolding procedure for the Polydimethylsiloxane (PDMS) double casting technique by introducing silicone oil as a non-adhesive barrier between PDMS layers. Unlike plasma or chemical surface modification methods, this approach requires no specialized or costly equipment, making it a more accessible and cost-effective...
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Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique10:11

Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique

Here, we present a protocol of heterotopic aortic transplantation in mice using the non-suture cuff technique in a cervical murine model. This model can be used to study the underlying pathology of chronic allograft vasculopathy (CAV) and can help evaluate new therapeutic agents in order to prevent its...
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Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications11:25

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

We describe key steps for biosensing by using polysilicon nanowire field-effect transistors, including the preparation of the device and the immobilization and confirmation of a DNA molecular probe on the nanowire surface, as well as conditions for DNA...
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