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Related Concept Videos

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry05:29

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

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A headspace solid-phase microextraction-gas-chromatography platform is described here for fast, reliable, and semi-automated volatile identification and quantification in ripe blackcurrant fruits. This technique can be used to increase knowledge about fruit aroma and to select cultivars with enhanced flavor for the purpose of breeding.
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Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry05:48

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This video presents a protocol for the mass spectrometrical analysis of volatile and oxidation sensitive compounds using electron impact ionization. The presented technique is especially of interest for inorganic chemists, working with metal organyls, silanes, or phosphanes which have to be handled using inert conditions, such as the Schlenk...
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Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry08:10

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Fizzy extraction is a new laboratory technique for analysis of volatile and semivolatile compounds. A carrier gas is dissolved in the liquid sample by applying overpressure and stirring the sample. The sample chamber is then decompressed. The analyte species are liberated to the gas phase due to...
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Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

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Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
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Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)17:12

Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)

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Capture Compounds are trifunctional small molecules to reduce the complexity of the proteome by functional reversible small molecule-protein interaction followed by photo-crosslinking and purification. Here we use a Capture Compound with S-adenosyl-L-homocysteine-binding as selectivity function to isolate methyltransferases from an Escherichia coli whole cell lysate and identify them by...
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Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry11:44

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry

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A two-dimensional gas chromatography-time-of-flight mass spectrometry method is described for characterization of the aqueous fraction of bio-crude produced from hydrothermal liquefaction of algae. This protocol can also be employed to analyze the aqueous fraction of liquid products from fast pyrolysis, catalytic fast pyrolysis, catalytic deoxygenation and hydro-treating.
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Related Experiment Video

Updated: Jan 20, 2026

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
05:29

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

Published on: June 9, 2021

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Volatile Organic Compound Profiling from Postmortem Microbes using Gas Chromatography-Mass Spectrometry.

Terezie Cernosek1, Kevin E Eckert1, David O Carter2

  • 1Laboratory of Forensic and Bioanalytical Chemistry, Forensic Sciences Unit, Division of Natural Sciences and Mathematics, Chaminade University of Honolulu, 3140 Waialae Avenue, Honolulu, HI.

Journal of Forensic Sciences
|September 4, 2019
PubMed
Summary

This study links specific volatile organic compounds (VOCs) to individual postmortem bacteria, identifying unique odor profiles from Bacillus subtilis and Ignatzschineria species. This research advances understanding of decomposition odor origins.

Keywords:
cadaver decompositiondecomposition odorforensic chemistryforensic scienceforensic taphonomypostmortem microbiologysolid-phase microextraction arrow

More Related Videos

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
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Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry
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Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry

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Related Experiment Videos

Last Updated: Jan 20, 2026

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
05:29

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

Published on: June 9, 2021

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Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
05:48

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry

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Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry
08:10

Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry

Published on: July 14, 2017

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Area of Science:

  • Forensic Science
  • Microbiology
  • Analytical Chemistry

Background:

  • Volatile organic compounds (VOCs) are key indicators of cadaveric decomposition and associated odors.
  • The specific microbial sources of these postmortem VOCs remain largely uncharacterized experimentally.

Purpose of the Study:

  • To profile VOCs emitted by three distinct postmortem bacterial isolates: Bacillus subtilis, Ignatzschineria indica, and Ignatzschineria ureiclastica.
  • To establish a direct experimental link between specific microbes and the VOCs they produce during decomposition.

Main Methods:

  • Utilized solid-phase microextraction arrow (SPME Arrow) for sample collection.
  • Employed gas chromatography-mass spectrometry (GC-MS) for precise VOC identification and quantification.
  • Monitored bacterial cultures (B. subtilis, I. indica, I. ureiclastica) over five days at 24°C on Standard Nutrient Agar.

Main Results:

  • Each bacterial species generated a unique VOC profile, including known decomposition compounds.
  • Observed temporal trends (upward or downward) in VOC production over the five-day period.
  • Identified significant production of dimethyldisulfide by Ignatzschineria indica, alongside alcohols, aldehydes, aromatics, and ketones.

Conclusions:

  • Provides foundational data connecting specific postmortem microbes to distinct VOC profiles.
  • Advances the understanding of the mechanisms driving decomposition odor production.
  • Offers potential for improved forensic investigations through microbial-VOC analysis.