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Updated: Jan 20, 2026
Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
Published on: March 2, 2020
Rational Design of a Dual-Reactivity-Based Fluorescent Probe for Visualizing Intracellular HSNO
Wei Chen1, Tetsuro Matsunaga2, Deshka L Neill1
1Department of Chemistry, Washington State University, Pullman, WA, 99164, USA.
Abstract:
Thionitrous acid (HSNO), the smallest S-nitrosothiol, is emerging as a potential key intermediate in cellular redox regulation linking two signaling molecules H2 S and NO. However, the chemical biology of HSNO remains poorly understood. A major hurdle is the lack of methods for selective detection of HSNO in biological systems. Herein, we report the rational design, synthesis, and evaluation of the first fluorescent probe TAP-1 for HSNO detection. TAP-1 showed high selectivity and sensitivity to HSNO in aqueous media and cells, providing a useful tool for understanding the functions of HSNO in biology.
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