The influence of Ca²⁺ concentration on voltage-dependent L-type calcium channels' expression in the marbled eel...

The catadromous species, eels, invariably exposed to variable Ca²⁺ concentrations circumstance i.e., lagoon or ocean. They need to maintain Ca²⁺ homeostasis by exchanging Ca²⁺ under different culture conditions. To understand the effects of environmental Ca²⁺ to fish, three types of genes coding for voltage-dependent L-type calcium channels (cacnb1, 2, 3) were cloned by screening an A. marmorata cDNA library. Tissue distribution analysis of Western blot showed that Cacnb1, 2, 3 had a significantly high expression in gill; while mRNA results showed the expressions of cacnb1 and cacnb3 were predominated in skin tissue but only cacnb2 was expressed in intestine. Serum osmolality and Ca²⁺ concentrations of A.marmorata were increased in a high calcium environment while reduced in a low calcium environment within 7 days; however, they were not significantly different among Ca²⁺ treatments after the eels were acclimated for 7 days. We also examined the influence of ambient Ca²⁺ levels on cacnbs expression of eels. With the increasing of exposure time, mRNA and protein expressions of cacnb1 were up-regulated in high level of Ca²⁺ (10 mM) and down-regulated in deficient Ca²⁺ (0 mM) compared to the control Ca²⁺ (2 mM). However, the opposite results were observed in cacnb2 and cacnb3. Notably, the cacnb2 expression was not significant different among Ca²⁺ treatments on day 7. Our study provided the insightful evidence that cacnbs play important roles in maintaining Ca²⁺ homeostasis of fish.