Related Experiment Video

Updated: Jan 20, 2026

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

9.2K

Towards Circulating-Tumor DNA-Based Precision Medicine

Ai Hironaka-Mitsuhashi1, Anna Sanchez Calle2, Takahiro Ochiya2,3

  • 1Division of Molecular and Cellular Medicine, National Cancer Centre Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan. ahironak@ncc.go.jp.

Journal of Clinical Medicine
|September 5, 2019
PubMed

Insights

Circulating-tumor DNAs (ctDNAs) offer a non-invasive way to analyze tumor genetics. This enables personalized cancer treatment decisions and monitoring, addressing unmet clinical needs in precision medicine.

Area of Science:

  • Oncology
  • Genomics
  • Molecular Diagnostics

Background:

  • Precision medicine relies on genomic information for targeted cancer therapies.
  • Advances in sequencing technologies enable analysis of circulating-tumor DNAs (ctDNAs).
  • ctDNAs provide molecular insights into individual tumors for treatment guidance.

Purpose of the Study:

  • To highlight the clinical applications of ctDNAs in cancer management.
  • To summarize studies on ctDNAs as predictive biomarkers for therapeutic response.
  • To explore ctDNA's role in the metastatic setting.

Main Methods:

  • Review of recent studies on ctDNA applications in oncology.
  • Analysis of ctDNA for molecular characterization of tumors.
  • Focus on ctDNA's utility in lung, breast, and colorectal cancers.

Main Results:

  • ctDNA analysis facilitates early diagnosis, prognosis, and biomarker identification.
  • ctDNAs are valuable for monitoring treatment response and clonal evolution.
  • Evidence supports ctDNAs as predictive biomarkers for therapy adaptation.

Conclusions:

  • ctDNAs are emerging as crucial tools in clinical oncology.
  • ctDNA analysis offers a promising approach to address unmet clinical needs.
  • ctDNAs are vital for guiding treatment decisions in precision oncology.
Keywords:
biomarkerbreast cancercirculating-tumor DNAcolorectal cancerlung cancerprecision medicinetargeted therapy

More Related Videos

Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH10:56

Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH

Published on: December 21, 2017

7.4K
Culture of Bladder Cancer Organoids as Precision Medicine Tools08:39

Culture of Bladder Cancer Organoids as Precision Medicine Tools

Published on: December 28, 2021

5.4K

Related Concept Videos

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Here, we present a protocol to detect tumor somatic mutations in circulating DNA present in patient biological fluids (biofluids). Our droplet digital polymerase chain reaction (dPCR)-based method enables quantification of the tumor mutation allelic frequency (MAF), facilitating a minimally invasive complement to diagnosis and temporal monitoring of tumor...
9.2K
Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH10:56

Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH

We present a new approach to characterize tumor cells. We combined immunofluorescence with DNA fluorescent-in-situ-hybridization to evaluate cells captured by a functionalized medical wire capable of in vivo enriching CTCs directly from patient blood.
7.4K
Culture of Bladder Cancer Organoids as Precision Medicine Tools08:39

Culture of Bladder Cancer Organoids as Precision Medicine Tools

Patient-derived organoids (PDOs) are a powerful tool in translational cancer research, reflecting both the genetic and phenotypic heterogeneity of the disease and response to personalized anti-cancer therapies. Here, a consolidated protocol to generate human primary bladder cancer PDOs in preparation for the evaluation of phenotypic analyses and drug responses is detailed.
5.4K
Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research07:47

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research

Culturing CTCs allows a deeper functional characterization of cancer, through assaying specific marker expression, and assessing drug resistance and the ability to colonize the liver among other possibilities. Overall, CTC culture could be a promising clinical tool for personalized medicine to improve patient outcome.
4.6K
Capture and Release of Viable Circulating Tumor Cells from Blood08:10

Capture and Release of Viable Circulating Tumor Cells from Blood

A protocol to utilize a poly(N-iso-propylacrylamide) (PIPAAm) coated microfilter for effective capture and thermoresponsive release of viable circulating tumor cells (CTC) is presented. This method allows capture of CTC from patients' blood and subsequent release of viable CTC for downstream off-chip culture, analyses and...
9.0K
Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model05:22

Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model

Circulating tumor cells (CTCs) have been shown to play an important role in tumor metastasis. Here, a method for the isolation and propagation of CTCs from the whole blood of a syngeneic mouse tumor model of hepatocellular carcinoma (HCC) metastasis is...
12.5K