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Related Concept Videos

Quantitation of Endothelial Cell Adhesiveness In Vitro10:24

Quantitation of Endothelial Cell Adhesiveness In Vitro

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We report an in vitro method that allows the quantitation of the actual number of adhesive cells within an endothelial cell monolayer.
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Cytoskeleton and Focal Adhesion Organization Assay: An Immunofluorescence-based Method to Study Cell Adhesion and Spreading on Substrates03:25

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This video describes the technique of studying the cytoskeleton and focal adhesions in primary human colon cancer cells to understand its variations depending on substrate rigidity. This culture of cancer cells on soft and hard substrates allows various downstream biophysical measurements for cancer...
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Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
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Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification09:11

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Here, we present a protocol to continuously quantify cell adhesion and de-adhesion processes with high temporal resolution in a non-invasive manner by cell-substrate impedance and live cell imaging analyses. These approaches reveal the dynamics of cell adhesion/de-adhesion processes triggered by matrix modification and their temporal relationship to adhesion-dependent signaling...
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We provide a method for isolating and culturing pure populations of heart valve endothelial cells (VEC). VEC can be isolated from either side of the cusp or leaflet and immediately following, underlying interstitial cell (VIC) isolation is straightforward.
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Related Experiment Video

Updated: Jan 20, 2026

Quantitation of Endothelial Cell Adhesiveness In Vitro
10:24

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Published on: June 18, 2015

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Focal adhesion clustering drives endothelial cell morphology on patterned surfaces.

C F Natale1,2, J Lafaurie-Janvore1, M Ventre2,3

  • 1Hydrodynamics Laboratory, Ecole Polytechnique, CNRS UMR7646, Palaiseau, France.

Journal of the Royal Society, Interface
|September 5, 2019
PubMed
Summary

Endothelial cell (EC) shape and alignment are crucial for vascular health. This study reveals that while both patterned adhesive and microgrooved surfaces guide EC orientation, cells elongate more on patterned adhesive surfaces due to focal adhesion clustering.

Keywords:
adhesive micropatternscell morphologycytoskeletonendothelial cellsfocal adhesionssubstrate topography

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Vascular Biology

Background:

  • Endothelial cell (EC) shape and alignment are critical for vascular function and disease development, particularly near blood flow disturbances.
  • Current in vitro models show ECs are sensitive to substrate properties, but mechanisms regulating their morphology and orientation remain unclear.
  • Substrate patterning, using adhesive motifs or topography, influences EC behavior, yet direct comparisons of their effects are limited.

Purpose of the Study:

  • To investigate how endothelial cells perceive and respond to patterned bio-adhesive versus microgrooved topographic surfaces with identical feature dimensions.
  • To elucidate the underlying cellular mechanisms, specifically focal adhesion clustering and cytoskeletal organization, that govern EC morphology and orientation on different patterned substrates.
  • To provide insights for designing improved biomaterials for cardiovascular devices.

Main Methods:

  • Fabrication of patterned surfaces with identical feature dimensions, comprising planar bio-adhesive motifs and microgrooved topographies.
  • Culturing of endothelial cells on these distinct patterned substrates.
  • Microscopic analysis to assess EC morphology, orientation, and focal adhesion distribution.

Main Results:

  • Both patterned bio-adhesive and microgrooved surfaces effectively guided and directed EC orientation.
  • Endothelial cells exhibited significantly greater elongation on patterned bio-adhesive surfaces compared to microgrooved surfaces.
  • Focal adhesion clustering was identified as the key regulatory factor for EC morphology, subsequently driving cytoskeletal organization.

Conclusions:

  • Substrate topography and adhesive patterning differentially regulate endothelial cell elongation.
  • Focal adhesion dynamics are central to mechanotransduction, controlling EC shape and cytoskeletal organization in response to surface cues.
  • Findings can inform the design of advanced biomaterials for enhancing the performance of implantable cardiovascular devices.