Sugar accumulation is associated with leaf senescence induced by long-term high light in wheat

During the grain filling stage, high light (HL) usually results in premature leaf senescence and significant yield loss in wheat. To explore the responses of sugar metabolism and the association of sugar accumulation and leaf senescence in HL, the activity and gene expression of sugar metabolism-related enzymes were analyzed when two wheat cultivars Triticum aestivum L. Xiaoyan 54 (XY54, HL tolerant) and Jing 411 (J411, HL sensitive) were transferred from low light (LL) to HL for 28 d. The results showed that the CO₂ assimilation rate, quantity of Rubisco and chlorophyll binding proteins decreased substantially for both cultivars in HL. However, the content of fructose, sucrose, and starch increased dramatically. In addition, the activity of hexokinase, pyruvate kinase, sucrose phosphate synthase, sucrose synthase, and alkaline/neutral invertase increased significantly while the expression of most of the sugar metabolism-related genes were repressed by long-term HL. Correlation analysis revealed that sugar content and sucrose phosphate synthase activity were negatively while the expression of most sugar metabolism-related genes were positively correlated with chlorophyll content during HL treatment. Comparatively, the HL tolerant cultivar XY54 accumulated less sugars than the HL sensitive cultivar J411, suggesting that sugar metabolism may be the regulation target for wheat improvement to cope with HL stress.