Related Experiment Video
Updated: Jan 20, 2026
Macromolecules: Identification Using Indicator Solutions - Concept
D-2-allylglycine embedded imidazolium-bridged polyhedral oligomeric silsesquioxane hybrid monolithic column for
Rui Chen1, Feng Zhao2, Xiang Li3
1Key Laboratory of Analytical Science and Technology of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China.
Abstract:
The development of multifarious stationary phases is still a growing demand so as to solve the tasks of ever evolving actual applications. Herein, with D-2-allylglycine hydrochloride (AG·HCl) as the hydrophilic monomer, diene ionic liquid 1-allyl-3-vinylimidazolium bromide (AVI·Br) and polyhedral oligomeric silsesquioxane methacryl substituted (POSS-MA) as the dual crosslinkers, the highly cross-linked imidazolium-bridged POSS-AVI-AG hybrid monolithic column was fabricated via the "one-pot" free radical copolymerization. The AG·HCl embedded POSS-AVI-AG column displays typical reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography mixed-mode retention mechanisms. Both hydrophobic phenols, alkylbenzenes, aromatic amines and hydrophilic nucleosides/nucleic acid bases, amides and thioureas were successfully separated with high column efficiencies (up to 571,000 plates/m for amides), outperforming our previously reported AVI·Br modified POSS-AVI column. Moreover, the column was also explored for the separation of cytochrome c tryptic digests and egg white protein extraction. All these results demonstrate that the POSS-AVI-AG column has a good potential in separation of both small molecules and complex biological samples with multiple mechanisms.
Related Concept Videos
06:26Macromolecules
Organisms contain a wide variety of organic molecules with numerous functions which depend on the chemical structures and properties of these molecules. All organic molecules contain a carbon backbone and hydrogen atoms. The carbon atom is central in the formation of a vast variety of organic molecules ranging in size, shape and complexity; inorganic molecules on the other hand, generally have simpler structures. The outermost shell of a free carbon atom can accommodate eight...
04:30Macromolecules
ExpandHYPOTHESES: In this activity, the experimental hypothesis might be that the addition of an indicator dye to each test solution will result in a color change if the test solution contains the macromolecule of interest. The null hypothesis might be that the addition of an indicator dye will not result in a color change in any of the test solutions.
Place 16 clean test tubes in a tube rack in four rows of four tubes and label each row A...
02:30Macromolecules
ExpandCAUTION: It is important to always use care when handling reagents. For example, iodine will stain clothes and skin so wear gloves, goggles, and a lab coat when using this reagent.
To prepare a 1 M glucose solution, begin by using a stir bar to dissolve 180.2 g of glucose in 500 mL of distilled water, increasing the volume to 1 L once the solute has dissolved in the smaller volume of solvent.
To prepare a 10% cornstarch solution, first use a hotplate to boil 1 L of...
06:44Curtain Flow Column: Optimization of Efficiency and Sensitivity
10:27Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
08:04Multifunctional Hybrid Fe2O3-Au Nanoparticles for Efficient Plasmonic Heating