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Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells07:53

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

We describe a method to evaluate the effect of pre-existing immunity against dengue virus on the Zika virus infection by using human serum, primary human cells, and infection quantification by quantitative real-time polymerase chain...
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A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum07:06

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

We describe a simple and easy protocol for measuring antibody dependent enhancement of infection by Zika virus convalescent serum using Dengue virus Reporter Viral...
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Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone07:35

Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone

This protocol describes the recovery of infectious Zika virus from a two-plasmid infectious cDNA...
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Vector Competence Analyses on Aedes aegypti Mosquitoes using Zika Virus10:35

Vector Competence Analyses on Aedes aegypti Mosquitoes using Zika Virus

The presented protocol can determine the vector competence of Aedes aegypti mosquito populations for a given virus, such as Zika, in a containment...
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Zika Virus Specific Diagnostic Epitope Discovery11:37

Zika Virus Specific Diagnostic Epitope Discovery

In this protocol, we describe a technique to discover Zika virus specific diagnostic peptides using a high-density peptide microarray. This protocol can readly be adapted for other emerging infectious...
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Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse10:31

Isolation and Quantification of Zika Virus from Multiple Organs in a Mouse

The goal of the protocol is to demonstrate the techniques used to investigate viral disease by isolating and quantifying Zika virus, from multiple organs in a mouse following...
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