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Related Concept Videos

Creating Matched In vivo/In vitro Patient-Derived Model Pairs of PDX and PDX-Derived Organoids for Cancer Pharmacology Research04:49

Creating Matched In vivo/In vitro Patient-Derived Model Pairs of PDX and PDX-Derived Organoids for Cancer Pharmacology Research

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A method is described to create organoids using patient-derived xenografts (PDX) for in vitro screening, resulting in matched pairs of in vivo/in vitro models. PDX tumors were harvested/processed into small pieces mechanically or enzymatically, followed by the Clevers’ method to grow tumor organoids that were passaged, cryopreserved and characterized against the original...
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Establishing Experimental Metastases Mouse Model: Implanting GFP Expressing CRC Organoid Cells in PDX Model to Detect Micrometastases03:24

Establishing Experimental Metastases Mouse Model: Implanting GFP Expressing CRC Organoid Cells in PDX Model to Detect Micrometastases

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This video describes the protocol for generating metastases by the PDX derived CRC organoids labeled with GFP lentivirus into the spleen of an immunodeficient mouse. Cancer micrometastases colonizing other organs can be detected using a fluorescence microscope to assess the GFP...
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Transduction to Label PDX Tumor Cells: Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro03:19

Transduction to Label PDX Tumor Cells: Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro

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The following video describes a technique of transduction to label (patient-derived xenografts) PDX tumor cells, which is  used for introducing lentivirus expressing green-fluorescent protein and luciferase reporters into the tumor cell in...
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In Ovo Xenografting of Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells (PDX-ALL)06:48

In Ovo Xenografting of Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells (PDX-ALL)

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This protocol describes in ovo xenografting of patient-derived B- and T-acute lymphoblastic leukemia (ALL) cells, which occurs 4 days following...
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Patient-derived Xenograft Modeling: A Technique to Generate Melanoma Mouse Models03:16

Patient-derived Xenograft Modeling: A Technique to Generate Melanoma Mouse Models

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In this video, human-derived melanoma cells are implanted subcutaneously into the flank region of an immunocompromised mouse. The PDX model allows preclinical investigation of melanoma cells that better recapitulate the tumor heterogeneity and melanoma aggressiveness observed in in vivo...
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A Melanoma Patient-Derived Xenograft Model07:07

A Melanoma Patient-Derived Xenograft Model

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Patient-derived xenograft (PDX) models more robustly recapitulate melanoma molecular and biological features and are more predictive of therapy response compared to traditional plastic tissue culture-based assays. Here we describe our standard operating protocol for the establishment of new PDX models and the characterization/experimentation of existing PDX...
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Related Experiment Video

Updated: Jan 20, 2026

Creating Matched In vivo/In vitro Patient-Derived Model Pairs of PDX and PDX-Derived Organoids for Cancer Pharmacology Research
04:49

Creating Matched In vivo/In vitro Patient-Derived Model Pairs of PDX and PDX-Derived Organoids for Cancer Pharmacology Research

Published on: May 5, 2021

7.0K

Know Thy PDX Model.

Terrence F Meehan1

  • 1Mouse Informatics Coordinator, European Bioinformatics Institute (EMBL-EBI), European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), European Molecular Biology Laboratory, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom. tmeehan@ebi.ac.uk.

Cancer Research
|September 5, 2019
PubMed
Summary
This summary is machine-generated.

Patient-derived tumor xenograft (PDX) models are validated as robust platforms for cancer research. The new Xenograft Visualization and Analysis (Xeva) software tool integrates drug response and genetic data for pharmacogenomic discovery.

More Related Videos

Establishing Experimental Metastases Mouse Model: Implanting GFP Expressing CRC Organoid Cells in PDX Model to Detect Micrometastases
03:24

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Transduction to Label PDX Tumor Cells: Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro
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Related Experiment Videos

Last Updated: Jan 20, 2026

Creating Matched In vivo/In vitro Patient-Derived Model Pairs of PDX and PDX-Derived Organoids for Cancer Pharmacology Research
04:49

Creating Matched In vivo/In vitro Patient-Derived Model Pairs of PDX and PDX-Derived Organoids for Cancer Pharmacology Research

Published on: May 5, 2021

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Establishing Experimental Metastases Mouse Model: Implanting GFP Expressing CRC Organoid Cells in PDX Model to Detect Micrometastases
03:24

Establishing Experimental Metastases Mouse Model: Implanting GFP Expressing CRC Organoid Cells in PDX Model to Detect Micrometastases

Published on: April 30, 2023

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Transduction to Label PDX Tumor Cells: Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro
03:19

Transduction to Label PDX Tumor Cells: Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro

Published on: April 30, 2023

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Area of Science:

  • Oncology
  • Bioinformatics
  • Pharmacogenomics

Background:

  • Patient-derived tumor xenograft (PDX) models are crucial for cancer research but face questions regarding clinical relevance due to tumor evolution differences.
  • Integrating diverse data types is essential for maximizing the utility of PDX models in translational cancer research.

Purpose of the Study:

  • To introduce the Xenograft Visualization and Analysis (Xeva) software tool for integrated pharmacogenomic analysis of PDX models.
  • To validate the robustness of PDX models for cancer treatment studies through a large-scale meta-analysis.

Main Methods:

  • Development and application of the Xeva software tool for integrating PDX tumor-drug response data with genetic information.
  • Conducting the largest meta-analysis of PDX models to date to assess their reliability in treatment studies.

Main Results:

  • Demonstration that PDX models are robust platforms for studying cancer mechanisms and therapeutic responses.
  • Xeva software facilitates comprehensive pharmacogenomic analysis by combining drug response and genetic data.

Conclusions:

  • PDX models serve as reliable systems for preclinical cancer research and drug development.
  • The Xeva tool enhances the potential for pharmacogenomic discovery by enabling integrated analysis of PDX data.