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Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae15:12

Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae

This video demonstrates the preparation of primary neuronal cultures from the brains of late stage Drosophila pupae. Views of live cultures show neurite outgrowth and imaging of calcium levels using...
15.7K
The Utility of Stage-specific Mid-to-late Drosophila Follicle Isolation08:44

The Utility of Stage-specific Mid-to-late Drosophila Follicle Isolation

Stage-specific isolation of mid-to-late Drosophila follicles is useful for a variety of purposes. Such follicles develop in culture, which allows for genetic and/or pharmacologic manipulations to be coupled with in vitro development assays and live imaging. Additionally, follicles can be used for molecular studies, such as isolating mRNA and...
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

We describe a technique for combining flow cytometry and high throughput sequencing to identify late replicating regions of the...
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Bioluminescence Imaging to Detect Late Stage Infection of African Trypanosomiasis07:59

Bioluminescence Imaging to Detect Late Stage Infection of African Trypanosomiasis

This manuscript describes the use of a bioluminescent strain of African trypanosomes to enable the tracking of late stage infection and demonstrates how in vivo live imaging can be used to visualize infections within the central nervous system in...
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Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad09:08

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

Here, we provide a dissection protocol required to live-image the late embryonic Drosophila male gonad. This protocol will permit observation of dynamic cellular processes under normal conditions or after transgenic or pharmacological...
3.6K
Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems06:06

Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems

Negative stain EM is a powerful technique for visualizing macromolecular structure, but different staining techniques can produce varying results in a sample dependent manner. Here several negative staining approaches are described in detail to provide an initial workflow for tackling the visualization of challenging...
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