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Related Experiment Video

Updated: Jan 20, 2026

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

Published on: January 19, 2024

High-throughput-compatible assays using a genetically-encoded calcium indicator.

Nyantsz Wu¹, Walter K Nishioka¹, Noël C Derecki¹

¹Janssen Research & Development, LLC, San Diego, CA, 92121, USA.

Scientific Reports

|September 5, 2019

Summary

This summary is machine-generated.

Genetically-encoded calcium indicators (GECIs) like GCaMP6s offer a cost-effective and simplified alternative to synthetic calcium indicators for live cell research. Studies show GCaMP6s performs comparably to synthetic indicators in drug discovery assays.

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Published on: November 19, 2014

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

Published on: April 1, 2011

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Last Updated: Jan 20, 2026

Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions

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Published on: January 19, 2024

Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

Published on: November 19, 2014

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

Published on: April 1, 2011

Area of Science:

Cellular Biology
Biochemistry
Pharmacology

Background:

Intracellular calcium measurement is vital for life science research and drug discovery.
Synthetic calcium indicators are widely used but can be costly and complex.
Genetically-encoded calcium indicators (GECIs) offer potential advantages in cost and assay simplification.

Purpose of the Study:

To compare the performance of a genetically-encoded calcium indicator (GECI), GCaMP6s, with a synthetic calcium indicator, fluo-4.
To evaluate GCaMP6s as a potential replacement for synthetic indicators in various assay formats.

Main Methods:

Generated a clonal cell line with stable, high expression of GCaMP6s using a blasticidin resistance gene and CHYSEL 2A peptide.
Compared the performance of GCaMP6s and fluo-4 in multiple assay formats.
Assessed the pharmacology of ion channel and GPCR ligands using both indicators.

Main Results:

GCaMP6s demonstrated high expression levels in the generated cell line.
The pharmacological profiles determined using GCaMP6s and fluo-4 were highly similar.
GCaMP6s proved viable as a direct replacement for fluo-4 in the tested assay formats.

Conclusions:

Genetically-encoded calcium indicators, specifically GCaMP6s, can effectively replace synthetic calcium indicators in many applications.
Stable expression of GECIs simplifies assays and reduces reagent costs.
GCaMP6s is a viable and effective tool for intracellular calcium measurement in drug discovery and basic research.