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A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis
Published on: April 10, 2019
Human Intestinal Enteroids Model MHC-II in the Gut Epithelium
Jonathan E Wosen1, Alexandra Ilstad-Minnihan1, Julia Y Co2
1Program in Immunology, Department of Pediatrics, Stanford University, Stanford, CA, United States.
Abstract:
The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, "mini-guts" derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel in vitro studies into IEC MHC-II regulation and function during health and disease.
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