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Updated: Jan 20, 2026

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes
Published on: September 17, 2012
Fluorescence In Situ Hybridization and Rehybridization Using Bacterial Artificial Chromosome Probes
Elzbieta Stankiewicz1, Tianyu Guo1, Xueying Mao1
1Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, London, UK.
Abstract:
Fluorescence in situ hybridization (FISH) method enables in situ genetic analysis of both metaphase and interphase cells from different types of material, including cell lines, cell smears, and fresh and paraffin-embedded tissue. Despite the growing number of commercially available FISH probes, still for large number of gene loci or chromosomal regions commercial probes are not available. Here we describe a simple method for generating FISH probes using bacterial artificial chromosomes (BAC). Due to genome-wide coverage of BAC clones, there are almost unlimited possibilities for the analysis of any genomic regions using BAC FISH probes.
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