Related Experiment Video
Updated: Jan 20, 2026
Anxiety Testing: Successive Alleys Test and Hyponeophagia Test
Published on: April 30, 2023
TMEM16B regulates anxiety-related behavior and GABAergic neuronal signaling in the central lateral amygdala
Ke-Xin Li1,2,3, Mu He1,2,3, Wenlei Ye1,2,3
1Department of Physiology, University of California, San Francisco, San Francisco, United States.
Abstract:
TMEM16B (ANO2) is the Ca2+-activated chloride channel expressed in multiple brain regions, including the amygdala. Here we report that Ano2 knockout mice exhibit impaired anxiety-related behaviors and context-independent fear memory, thus implicating TMEM16B in anxiety modulation. We found that TMEM16B is expressed in somatostatin-positive (SOM+) GABAergic neurons of the central lateral amygdala (CeL), and its activity modulates action potential duration and inhibitory postsynaptic current (IPSC). We further provide evidence for TMEM16B actions not only in the soma but also in the presynaptic nerve terminals of GABAergic neurons. Our study reveals an intriguing role for TMEM16B in context-independent but not context-dependent fear memory, and supports the notion that dysfunction of the amygdala contributes to anxiety-related behaviors.
Related Concept Videos
Anxiety Testing
04:18Visualization and Mapping of Neuronal Pathways in an Amygdala Brain Slice
07:12Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice
02:05Generating a Coronal Brain Slice Exposing the Amygdala Regions from a Mouse Brain
03:01Calcium Imaging in Individual Neurons Regulating Egg-Laying Behavior in Caenorhabditis elegans
08:17Reducing State Anxiety Using Working Memory Maintenance

