Flavobacterium ranwuense sp. nov., isolated from glacier

A Gram-stain-negative, aerobic, rod-shaped, devoid of flagella, orange-yellow-coloured bacterium (strain LB2P22^T) was isolated from an ice sample collected from the Laigu glacier on the Tibetan Plateau, PR China. The results of 16S rRNA gene sequence analysis indicated that strain LB2P22^T belongs to the genus Flavobacterium with highest similarity to Flavobacterium laiguense LB2P30^T (98.14 %), Flavobacterium glaciei CGMCC 1.5380^T (98.02 %), Flavobacterium psychroterrae CCM 8827^T (97.94 %) and Flavobacterium granuli CGMCC 1.10125^T (97.91 %). Strain LB2P22^T had 77.99-84.99 % average nucleotide identity and 22.3-29.3 % digital DNA-DNA hybridization values with its closest relatives, indicating that it represents a novel species of the genus Flavobacterium. Strain LB2P22^T grew at 0-25 °C, pH 7.0-8.0 and up to 1.0 % (w/v) NaCl. The main cellular fatty acids were iso-C_15 : 0, summed feature 3 (comprising C_16 : 1ω7c/C_16 : 1ω6c) and anteiso-C_15 : 0. The genomic DNA G+C content is 34.3 mol%. The predominant menaquinone was MK-6 and the polar lipids were phosphatidylethanolamine, one unidentified aminolipid and four unidentified lipids. Based on these data, a novel species, Flavobacteriumranwuense sp. nov., is proposed, with LB2P22^T (=CGMCC 1.11361^T=NBRC 113777^T) as the type strain.