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Related Concept Videos

Plaquing of Herpes Simplex Viruses04:41

Plaquing of Herpes Simplex Viruses

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Plaquing is a routine method used to quantify live viruses in a population. Though plaquing is frequently taught in various microbiology curricula with bacteria and bacteriophages, plaquing of mammalian viruses is more complex and time-consuming. This protocol describes the procedures that function reliably for regular work with herpes simplex...
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In this manuscript, we describe a simple method of growth, purification, and titration of the oncolytic herpes simplex virus for preclinical use.
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Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 111:56

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The skin is one target tissue of the human pathogen herpes simplex virus type 1 (HSV-1). To explore the invasion route of HSV-1 into tissue, we established an ex vivo infection model of murine epidermal sheets which represent the outermost layer of...
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Virus Plaque Assay: A Technique to Measure Infectious Herpes Simplex Virus via Quantification of Plaques Formed in Monolayer of Virus-Infected Cells03:43

Virus Plaque Assay: A Technique to Measure Infectious Herpes Simplex Virus via Quantification of Plaques Formed in Monolayer of Virus-Infected Cells

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This video describes the virus plaque assay of the infectious herpes simplex virus or HSV. The diluted viral particles are inoculated to the monolayer of epithelial cells. Upon incubation, the virus forms plaques and is counted visually to determine the infectious virus...
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siRNA-Based Inhibition of Autophagy in Herpes Simplex Virus-Infected Immature Dendritic Cells03:44

siRNA-Based Inhibition of Autophagy in Herpes Simplex Virus-Infected Immature Dendritic Cells

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This video demonstrates a small interfering RNA (siRNA)-based technique to interfere with autophagic flux. A siRNA targeting FIP200 mRNA is introduced into monocyte-derived immature dendritic cells (iDCs) using electroporation, causing the knockdown of FIP200 expression and leading to the inhibition of nuclear lamin disruption via autophagy. Upon adding herpes simplex virus (HSV), the nuclear egress of viral progeny is inhibited due to the absence of lamin disruption via autophagic...
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Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy06:40

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

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ICP0 undergoes nuclear-to-cytoplasmic translocation during HSV-1 infection. The molecular mechanism of this event is not known. Here we describe the use of confocal microscope as a tool to quantify ICP0 movement in HSV-1 infection, which lays the groundwork for quantitatively analyzing ICP0 translocation in future mechanistic...
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Related Experiment Video

Updated: Jan 20, 2026

Plaquing of Herpes Simplex Viruses
04:41

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Herpes Simplex Virus Keratitis.

Karen D Harris1

  • 1Karen D. Harris, MSN, RN, ACNS-BC, is an Assistant Professor, McAuley School of Nursing, University of Detroit Mercy, Detroit, Michigan.

Home Healthcare Now
|September 5, 2019
PubMed
Summary
This summary is machine-generated.

Herpes simplex virus (HSV) keratitis is a leading cause of corneal blindness in the US. Early detection by home care clinicians recognizing HSV signs and symptoms is crucial for timely treatment and prevention.

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Last Updated: Jan 20, 2026

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Area of Science:

  • Ophthalmology
  • Infectious Diseases
  • Virology

Background:

  • Herpes simplex virus (HSV) is a widespread infection in the United States.
  • HSV keratitis, an ocular manifestation of HSV, is a primary cause of corneal blindness domestically.
  • Early identification and intervention are critical for managing this serious condition.

Purpose of the Study:

  • To inform home care clinicians about the signs and symptoms of HSV keratitis.
  • To emphasize the role of early detection in managing HSV keratitis.
  • To provide an overview of preventive measures and treatment options for HSV keratitis.

Main Methods:

  • This article reviews the clinical presentation of HSV keratitis.
  • It discusses diagnostic indicators for HSV infection of the eye.
  • Preventive strategies and therapeutic interventions are examined.

Main Results:

  • Knowledge of HSV and HSV keratitis signs aids early detection.
  • Prompt treatment can mitigate vision loss.
  • Preventive measures can reduce recurrence.

Conclusions:

  • Home care clinicians play a vital role in the early diagnosis of HSV keratitis.
  • Understanding HSV keratitis is essential for preventing corneal blindness.
  • Comprehensive management includes prevention and timely treatment.