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Mouse Cardiac Arrest Model for Brain Imaging and Brain Physiology Monitoring During Ischemia and Resuscitation07:18

Mouse Cardiac Arrest Model for Brain Imaging and Brain Physiology Monitoring During Ischemia and Resuscitation

This protocol demonstrates a unique mouse model of asphyxia cardiac arrest that does not require chest compression for resuscitation. This model is useful for monitoring and imaging the dynamics of brain physiology during cardiac arrest and...
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Rodent Brain Microinjection Using a Quartz Microneedle02:58

Rodent Brain Microinjection Using a Quartz Microneedle

Source: Paolone, G., et al. Personalized Needles for Microinjections in the Rodent Brain. J. Vis. Exp. (2018).This video demonstrates a rodent brain microneedle injection using a quartz microneedle with holes. The microneedle is attached to a syringe and micropump to control the delivery rate. The needle with the injection solution is inserted into the brain with the delivery speed set at low. Once positioned, the delivery speed is increased. The needle is withdrawn after a brief...
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Standardized Model of Ventricular Fibrillation and Advanced Cardiac Life Support in Swine05:36

Standardized Model of Ventricular Fibrillation and Advanced Cardiac Life Support in Swine

Cardiopulmonary resuscitation and defibrillation are the only effective therapeutic options during cardiac arrest caused by ventricular fibrillation. This model presents a standardized regimen to induce, assess, and treat this physiological state in a porcine model, thus providing a clinical approach with various opportunities for data collection and...
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Advanced Diffusion Imaging in The Hippocampus of Rats with Mild Traumatic Brain Injury10:33

Advanced Diffusion Imaging in The Hippocampus of Rats with Mild Traumatic Brain Injury

The overall goal of this procedure is to obtain quantitative microstructural information of the hippocampus in a rat with mild traumatic brain injury. This is done using an advanced diffusion-weighted magnetic resonance imaging protocol and region-of-interest based analysis of parametric diffusion...
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Lavage-induced Surfactant Depletion in Pigs As a Model of the Acute Respiratory Distress Syndrome (ARDS)07:20

Lavage-induced Surfactant Depletion in Pigs As a Model of the Acute Respiratory Distress Syndrome (ARDS)

Repeated pulmonary lavages in anesthetized pigs induce lung injury resembling major aspects of human acute respiratory distress syndrome (ARDS). For this purpose the lungs are repeatedly lavaged with 0.9% saline at 37 °C. The goal of the protocol is a reproducible mitigation of gas exchange and hemodynamics for research in...
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Magnetic Resonance-Guided Stereotaxy for Infusions to the Pig Brain08:23

Magnetic Resonance-Guided Stereotaxy for Infusions to the Pig Brain

The protocol presented here demonstrates stereotaxy to the pig brain using convection-enhanced infusions, with real-time magnetic resonance imaging (MRI) visualization guidance and real-time infusion distribution visualization.
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