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A Multiple Integrated Social Stress Model for Psychiatric Disorders in Female C57BL/6J Mice06:15

A Multiple Integrated Social Stress Model for Psychiatric Disorders in Female C57BL/6J Mice

By integrating four daily social stressors, the present study describes a multiple integrated social stress (MISS) model for psychiatric disorders in female C57BL/6J mice. After ten days of repeated MISS exposure, the mice developed depressive- and anxiety-like phenotypes, thus providing a natural, etiology-based animal model for studying psychiatric disorders in female...
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5-Methylcytosine Dot Blot to Determine the Extent of DNA Methylation04:11

5-Methylcytosine Dot Blot to Determine the Extent of DNA Methylation

This video describes the technique of dot-blot to determine the level of DNA methylation in human chondrocytes in vitro via the blotting of DNA samples on a nylon membrane and the subsequent visualization using monoclonal antibodies. This helps to determine the chondrocytes' phenotype at various stages in...
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Developing a Behavioral Box for Assessing Prepulse Inhibition and Neural Activity in Psychiatric Animal Models06:55

Developing a Behavioral Box for Assessing Prepulse Inhibition and Neural Activity in Psychiatric Animal Models

This protocol presents a microcontroller-based behavioral box to assess prepulse inhibition (PPI) by collecting acceleration data from a sensor beneath the box. This data evaluates sensory gating deficits in socially isolated rats, with an added method for synchronizing data with neuronal activity to advance neurophysiology studies on behavioral...
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Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons10:11

Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons

An easy and convenient method to determine the extent of gap junction tracer coupling between retinal neurons is described. This technique enables one to investigate the function of the electrical synapses between neurons in the intact retina under different illumination conditions and at different times of the day and...
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International Expert Consensus and Recommendations for Neonatal Pneumothorax Ultrasound Diagnosis and Ultrasound-guided Thoracentesis Procedure05:50

International Expert Consensus and Recommendations for Neonatal Pneumothorax Ultrasound Diagnosis and Ultrasound-guided Thoracentesis Procedure

Pneumothorax is a common emergency and critical disease in newborn infants that needs rapid, clear diagnosis and timely treatment. Diagnosis and treatment based on chest X-rays are associated with delayed management and radiation damage. Lung ultrasound (US) provides useful guidance for rapid, accurate diagnosis and the precise thoracentesis of...
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Protocol and Guidelines for Point-of-Care Lung Ultrasound in Diagnosing Neonatal Pulmonary Diseases Based on International Expert Consensus06:15

Protocol and Guidelines for Point-of-Care Lung Ultrasound in Diagnosing Neonatal Pulmonary Diseases Based on International Expert Consensus

Lung ultrasound is a noninvasive and valuable tool for bedside evaluation of neonatal lung diseases. However, a relative lack of reference standards, protocols and guidelines may limit its application. Here, we aim to develop a standardized neonatal lung ultrasound diagnostic protocol to be used in clinical...
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