Methylobacterium oryzihabitans sp. nov., isolated from water sampled from a rice paddy field

Strain TER-1^T was isolated from water sampled from a rice paddy field in Taiwan. Cells were Gram-negative, aerobic, motile, rod-shaped and formed pink-coloured colonies. Optimal growth occurred at 25-30 °C, pH 6-7 and in the presence of 0.5 % NaCl. Strain TER-1^T could grow on C1 compounds such as methanol, formic acid, methylamine and dimethylamine as sole carbon source, and carry methanol dehydrogenase gene, which supports its methylotrophic metabolism. The results of phylogenetic analyses based on the 16S rRNA gene sequence, methanol dehydrogenase gene sequence and coding sequences of 92 protein clusters indicated that strain TER-1^T formed a phylogenetic lineage in the genus Methylobacterium. Strain TER-1^T was most closely related to Methylobacterium isbiliense AR24^T with 96.8 % 16S rRNA gene sequence similarity. Strain TER-1^T showed 77.1-82.8 % average nucleotide identity and 16.4-20.2 % digital DNA-DNA hybridization identity with the strains of other Methylobacterium species. The major fatty acid of strain TER-1^T was C_18 : 1ω7c. The predominant hydroxy fatty acid was C_18 : 0 3-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and two uncharacterized lipids. The only isoprenoid quinone was Q-10. The genomic DNA G+C content of strain TER-1^T was 71.9 mol%. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TER-1^T should be classified in a novel species of the genus Methylobacterium, for which the name Methylobacterium oryzihabitans sp. nov. is proposed. The type strain is TER-1^T (=BCRC 81157^T=LMG 30931^T=KCTC 62864^T).