[Application of NASBA and RPA in detection of pathogenic bacteria]

Y Yue1, J Z Zhang2, M J Zhang2

  • 1Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Ningxia Hui Autonomous Region Food Testing and Research Institute, Yinchuan 750001, China.

Related Concept Videos

Immunohistochemical Detection of Pathogenic Bacteria in Human Brain Tissue01:18

Immunohistochemical Detection of Pathogenic Bacteria in Human Brain Tissue

To detect these bacteria, take a deparaffinized, rehydrated, bacteria-infected human brain section with exposed protein antigens and inactivated endogenous...
153
On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System07:46

On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System

Here, we describe a simple and quick procedure for detecting DNA from bee pathogens, such as Lotmaria passim and Nosema ceranae, using an amplification-ready cell lysis, recombinase polymerase amplification, and CRISPR/Cas12a...
872
DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

We present a protocol that combines recombinase polymerase amplification with a CRISPR/Cas12a system for trace detection of DNA viruses and builds portable smartphone microscopy with an artificial intelligence-assisted classification for point-of-care DNA virus...
1.5K
Colorimetric Detection of Bacteria Using Litmus Test10:05

Colorimetric Detection of Bacteria Using Litmus Test

We describe a protocol for colorimetric detection of E. coli using a modified litmus test that takes advantage of an RNA-cleaving DNAzyme, urease, and magnetic beads.
9.8K
An In Vitro Technique to Stimulate Lymphocytes via Pathogenic Bacteria03:23

An In Vitro Technique to Stimulate Lymphocytes via Pathogenic Bacteria

This video demonstrates an in vitro technique to stimulate lymphocytes via non-typeable Haemophilus influenzae (NTHi) and to assess the immune response. Incubating isolated peripheral blood mononuclear cells (PBMCs) with NTHi causes the bacterial cells to stimulate T lymphocytes, leading to cytokine production. Upon inhibiting the secretion of cytokines, immunofluorescence staining is performed to detect the accumulated intracellular cytokines in stimulated T...
499
Detection of Foodborne Bacterial Pathogens from Individual Filth Flies12:54

Detection of Foodborne Bacterial Pathogens from Individual Filth Flies

A PCR-based protocol was adapted to detect Cronobacter spp., Salmonella enterica, and Listeria monocytogenes from body surfaces and alimentary canals of individual wild-caught flies. The goal of this protocol is to detect and isolate bacterial pathogens from individual insects collected as part of an environmental sampling program during foodborne outbreak...
18.9K