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Updated: Jan 20, 2026

Immunohistochemical Detection of Pathogenic Bacteria in Human Brain Tissue
[Application of NASBA and RPA in detection of pathogenic bacteria]
Y Yue1, J Z Zhang2, M J Zhang2
1Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Ningxia Hui Autonomous Region Food Testing and Research Institute, Yinchuan 750001, China.
Abstract:
Nucleic acid sequence-based amplification and recombinase polymerase amplification are the recently developed thermostatic amplification techniques based on PCR. This paper briefly summarizes the principle of reaction, design principle of primer and probe, advantage of these two techniques (simple, accurate, highly sensitive and rapid) and introduces the application of the techniques in the detection of pathogenic bacteria.
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