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Related Concept Videos

Identification of Post-translational Modifications of Plant Protein Complexes10:07

Identification of Post-translational Modifications of Plant Protein Complexes

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We describe here a protocol for the purification and characterization of plant protein complexes. We demonstrate that by immunoprecipitating a single protein within a complex, so we can identify its post-translational modifications and its interacting...
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Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications10:12

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The present protocol highlights a modified method to detect and quantify DNA-protein crosslinks (DPCs) and their post-translational modifications (PTMs), including ubiquitylation, SUMOylation, and ADP-ribosylation induced by topoisomerase inhibitors and by formaldehyde, thereby allowing the study of the formation and repair of DPCs and their PTMs.
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Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications09:22

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The preparation of high quality yeast cell extracts is a necessary first step in the analysis of individual proteins or entire proteomes. Here we describe a fast, efficient, and reliable homogenization protocol for budding yeast cells that has been optimized to preserve protein functions, interactions, and post-translational...
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Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations10:26

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Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches05:56

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The present protocol uses a biomolecular simulation package and describes the molecular dynamics (MD) approach for modeling the wild-type caspase and its mutant forms. The MD method allows for assessing the dynamic evolution of the caspase structure and the potential effect of mutations or post-translational...
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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications09:29

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Related Experiment Video

Updated: Jan 20, 2026

Identification of Post-translational Modifications of Plant Protein Complexes
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Protein post-translational modifications in bacteria.

Boris Macek1, Karl Forchhammer2, Julie Hardouin3

  • 1Proteome Center Tübingen, University of Tübingen, Tübingen, Germany. boris.macek@uni-tuebingen.de.

Nature Reviews. Microbiology
|September 6, 2019
PubMed
Summary
This summary is machine-generated.

Bacterial protein post-translational modifications are increasingly found, playing key roles in cellular functions and disease. Understanding these modifications is crucial for advancing bacterial physiology and developing new treatments for infections.

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Related Experiment Videos

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Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications
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Area of Science:

  • Microbiology
  • Biochemistry
  • Molecular Biology

Background:

  • The study of bacterial protein post-translational modifications (PTMs) has expanded significantly over the last decade.
  • Bacterial PTMs are often substoichiometric and affect a limited number of proteins compared to eukaryotes, posing analytical challenges.
  • The prevalence of PTMs varies across bacterial species and is influenced by environmental factors.

Purpose of the Study:

  • To review the current understanding of bacterial protein post-translational modifications.
  • To highlight the functional significance of bacterial PTMs in various cellular processes.
  • To emphasize the potential of PTM research for understanding bacterial physiology and developing novel therapeutic strategies.

Main Methods:

  • Literature review and synthesis of existing research on bacterial PTMs.
  • Analysis of the structural and functional implications of PTMs in bacterial systems.
  • Discussion of the impact of environmental conditions on the bacterial modified proteome.

Main Results:

  • Evidence indicates that bacterial PTMs are vital for processes including protein synthesis, nitrogen metabolism, cell cycle regulation, dormancy, sporulation, and virulence.
  • The number of enzymes responsible for PTMs varies considerably among different bacterial species.
  • Substoichiometric modification levels present significant challenges for structural and functional characterization.

Conclusions:

  • Protein post-translational modifications are essential regulatory mechanisms in bacteria with broad physiological roles.
  • Further investigation into bacterial PTMs is critical for a comprehensive understanding of bacterial life.
  • Targeting bacterial PTMs offers promising new avenues for the development of treatments against infectious diseases.