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The protocol describes a method for creating a patient-derived colorectal cancer (CRC) organoid model co-cultured with tumor-infiltrating lymphocytes (TILs) to study their interactions and therapeutic potential. This model provides a preclinical platform to explore immune responses in the tumor microenvironment and to predict the efficacy of TIL-based therapy for personalized CRC...
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We describe a method to utilize tumor-infiltrating lymphocytes (TILs) from mice through flow cytometry for adoptive cell transfer. This protocol aims to verify the specific cytotoxicity of TILs against tumors in a syngeneic pancreatic cancer mouse model, providing insights into the development of adoptive cell therapies for pancreatic...
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