Cerebrospinal Fluid (CSF) Analysis and Interpretation in Neurocritical Care for Acute Neurological Conditions

Ajay Prasad Hrishi1, Manikandan Sethuraman1

  • 1Department of Anaesthesiology, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India.

Related Concept Videos

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid10:23

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

A method for mass spectrometric analysis of endogenous peptides in human cerebrospinal fluid (CSF) is presented. By employing molecular weight cut-off filtration, chromatographic pre-fractionation, mass spectrometric analysis and a subsequent combination of peptide identification strategies, it was possible to expand the known CSF peptidome nearly ten-fold compared to previous...
9.4K
An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice06:40

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal...
66.2K
Immunoassay to Detect Age-Related Neurogenesis from Cerebrospinal Fluid01:28

Immunoassay to Detect Age-Related Neurogenesis from Cerebrospinal Fluid

Centrifuge the sample to pellet cellular debris and collect the...
160
Cerebrospinal Fluid01:21

Cerebrospinal Fluid

Cerebrospinal fluid (CSF) is a colorless liquid that flows around the brain and the spinal cord, playing a vital role in the protection, support, and overall function of the central nervous system (CNS). CSF production, circulation, and absorption are tightly regulated processes essential for the brain and spinal cord to function properly.
CSF Production
CSF is produced mainly in the choroid plexus, a network of capillaries and ependymal cells located within the ventricular system of the brain....
5.4K
Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid06:21

Osmotic Minipump Implantation for Increasing Glucose Concentration in Mouse Cerebrospinal Fluid

This article describes a detailed protocol to increase glucose concentration in the cerebrospinal fluid (CSF) of mice. This approach can be useful for studying the effects of high CSF glucose on neurodegeneration, cognition, and peripheral glucose metabolism in...
2.0K
In Vivo Imaging of Cerebrospinal Fluid Transport in a Mouse Brain Using Fluorescence Macroscopy02:41

In Vivo Imaging of Cerebrospinal Fluid Transport in a Mouse Brain Using Fluorescence Macroscopy

Source: Sweeney, A. M., et al. In Vivo Imaging of Cerebrospinal Fluid Transport through the Intact Mouse Skull using Fluorescence Macroscopy. J. Vis. Exp. (2019)This video demonstrates the process of visualizing cerebrospinal fluid (CSF) flow in a mouse brain using fluorescence imaging. A fluorescent tracer is injected into the cisterna magna (CM), allowing its movement with CSF to be tracked through the subarachnoid and perivascular spaces. Time-lapse imaging captures the tracer's journey,...
615