Modelling Cell Origami via a Tensegrity Model of the Cytoskeleton in Adherent Cells

Area of Science:

• Biotechnology
• Cellular Mechanics
• Finite Element Analysis

Background:

• Cell origami is a valuable technique for creating 3D cell-populated microstructures.
• It offers advantages like biocompatibility and minimal cell damage.

Purpose of the Study:

• To develop a 3D finite element method (FEM) model of adherent cells using tensegrity structures.
• To investigate the influence of nucleoskeleton (NSK) and tensegrity complexity on microplate folding angles.

Main Methods:

• Constructed a 3D FEM model of an adherent cell incorporating cytoskeleton (CSK) and nucleoskeleton (NSK).
• Modeled focal adhesion complexes (FACs) using spring elements connecting the cell to a microplate.
• Established four models varying NSK inclusion and tensegrity complexity.

Main Results:

• The nucleoskeleton (NSK) inclusion increased the microplate folding angle, simulating a more physiological cellular environment.
• Increased tensegrity structure complexity reduced the folding angle, dependent on cell type.
• FEM models were validated against existing data.

Conclusions:

• The study provides a validated FEM model for cell origami applications.
• Findings offer theoretical guidance for biotechnology and 3D cell structure analysis.
• Implications for the self-assembly of cell-based microscale medical devices.