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Related Concept Videos

An Affinity Chromatography Technique for the Purification of a Recombinant Bacterial Protein02:54

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Source: Becker, B., et al. A Simple Fluorescence-based Reporter Assay to Identify Cellular Components Required for Ricin Toxin A Chain (RTA) Trafficking in Yeast. J. Vis. Exp. (2017).This video demonstrates the purification of a polyhistidine-tagged recombinant protein using nickel-based affinity chromatography. It outlines key steps, including bacterial lysis by sonication, clarification and filtration of the lysate, and selective binding and elution of the target protein using...
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Updated: Jan 20, 2026

An Affinity Chromatography Technique for the Purification of a Recombinant Bacterial Protein
02:54

An Affinity Chromatography Technique for the Purification of a Recombinant Bacterial Protein

Published on: November 28, 2025

184

Aptamer-Based Affinity Chromatography for Protein Extraction and Purification.

G Perret1, E Boschetti2

  • 1LFB Biotech, Les Ulis, France. perret@netcourrier.com.

Advances in Biochemical Engineering/Biotechnology
|September 6, 2019
PubMed
Summary
This summary is machine-generated.

Aptamers, or oligonucleotide molecules, offer effective protein separation via affinity chromatography. While promising, large-scale applications require further optimization and validation for widespread use.

Keywords:
Affinity chromatographyAptamerProtein purification

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Area of Science:

  • Biochemistry
  • Biotechnology
  • Analytical Chemistry

Background:

  • Aptamers are oligonucleotide molecules with high specificity for protein recognition.
  • Aptamers offer advantages over existing protein separation technologies in affinity chromatography.
  • Previous applications have demonstrated the potential of aptamers in protein separation.

Purpose of the Study:

  • To discuss the context and applications of aptamer ligands in affinity chromatography.
  • To present chemical modifications of aptamers for covalent coupling and separation processes.
  • To compare aptamer-based chromatography with immunoaffinity chromatography for protein separation.

Main Methods:

  • Adaptation of the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) process for aptamer selection.
  • Chemical modifications of aptamers to enable covalent coupling to chromatography supports.
  • Application of aptamer ligands in affinity chromatography for protein separation.

Main Results:

  • Aptamers demonstrate effective protein separation with advantages over current technologies.
  • Detailed presentation of aptamer chemical modifications for chromatographic applications.
  • Real-life examples highlight the utility of aptamer-based protein separation.

Conclusions:

  • Aptamer-based affinity chromatography shows extraordinary potential for protein separation.
  • Significant optimization and validation are needed for large-scale industrial applications.
  • Further research is required to overcome limitations for widespread adoption.