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Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties
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Multi-bioactive silver nanoparticles synthesized using mosquitocidal Bacilli and their characterization.

Magda A El-Bendary1, Maysa E Moharam2, Mohamed Abdelraof2

  • 1Genetic Engineering and Biotechnology Research Division, Microbial Chemistry Department, National Research Centre (Scopus ID: 60014618), 33 Bohouth Street, Dokki, Giza, Egypt. tasnim41@yahoo.com.

Archives of Microbiology
|September 6, 2019
PubMed
Summary

Mosquitocidal bacteria were used to synthesize multi-bioactive silver nanoparticles (AgNPs). These eco-friendly AgNPs show potent antimicrobial and insecticidal properties with low cytotoxicity, suggesting medical applications.

Keywords:
BactericidalBiogenic silver nanoparticlesCytotoxicityFungicidalMosquitocidalVirucidal

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Area of Science:

  • Nanotechnology
  • Microbiology
  • Materials Science

Background:

  • Silver nanoparticles (AgNPs) are increasingly explored for their diverse applications.
  • Biosynthesis offers a sustainable route for nanoparticle production.
  • Mosquitocidal bacteria present a potential source for novel bioactive agents.

Purpose of the Study:

  • To synthesize silver nanoparticles (AgNPs) using cell-free filtrates of mosquitocidal Bacilli.
  • To characterize the synthesized AgNPs and investigate their bioactive properties.
  • To explore the potential of these AgNPs in medical and pest control applications.

Main Methods:

  • Synthesis of AgNPs using bacterial filtrates.
  • Characterization using UV-Vis spectroscopy, Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS), and Fourier-Transform Infrared Spectroscopy (FTIR).
  • Evaluation of mosquitocidal, bactericidal, fungicidal, virucidal, and cytotoxic activities.

Main Results:

  • AgNPs synthesized with optical absorption peaks at 386-412 nm.
  • TEM revealed polycrystalline spherical, hexagonal, cuboidal, rod, and anisotropic shapes; DLS indicated sizes of 15-21 nm.
  • FTIR confirmed protein capping, suggesting stabilization by nitrate reductase activity.
  • AgNPs demonstrated broad bioactivity (mosquitocidal, bactericidal, fungicidal, virucidal) with low cytotoxicity to MA 104 cells.

Conclusions:

  • Cell-free filtrates of mosquitocidal Bacilli are effective for synthesizing multi-bioactive AgNPs.
  • Nitrate reductase likely plays a role in AgNP formation and stabilization.
  • Synthesized AgNPs exhibit significant antimicrobial and insecticidal effects with potential for medical and agricultural applications.
  • This study presents an eco-friendly, economic method for producing functional AgNPs.