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Updated: Jan 20, 2026
Targeting Liver Tumors with Oncolytic Viruses via the Hepatic Artery
A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death
Tiphaine Delaunay1,2, Carole Achard1,2, Marc Grégoire1,2
1CRCINA, INSERM, Université d'Angers, Université de Nantes, Nantes, France.
Abstract:
Oncolytic immunotherapy efficacy relies partially on the induction of immunogenic tumor cell death following infection with oncolytic viruses (OV) to induce an antitumor immune response. Here, we describe a method to determine if an OV is able to induce such an immunogenic tumor cell death. This method consists in testing whether tumor cells lysed by an OV are able to induce the maturation of human monocyte-derived immature dendritic cells (Mo-iDC).
Insights
We developed a new method to assess if oncolytic viruses (OV) cause immunogenic tumor cell death. This involves testing if tumor cells killed by OV can mature dendritic cells, crucial for antitumor immune responses.
Area of Science:
- Immunology
- Virology
- Cancer Therapy
Background:
- Oncolytic immunotherapy efficacy depends on inducing immunogenic tumor cell death.
- Oncolytic viruses (OV) are a promising cancer treatment strategy.
- Assessing OV-induced immunogenic cell death is critical for therapeutic development.
Purpose of the Study:
- To establish a method for determining if an oncolytic virus (OV) induces immunogenic tumor cell death.
- To evaluate the potential of OV in stimulating an antitumor immune response.
Main Methods:
- Developing a novel assay to assess OV efficacy.
- Testing the ability of OV-lysed tumor cells to induce maturation of human monocyte-derived immature dendritic cells (Mo-iDC).
Main Results:
- The described method allows for the evaluation of OV-induced immunogenic tumor cell death.
- This assay can determine if tumor cells killed by OV can effectively stimulate dendritic cell maturation.
Conclusions:
- This method provides a reliable way to assess a key mechanism of oncolytic immunotherapy.
- It aids in the selection and development of effective oncolytic viruses for cancer treatment.
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