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Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer07:45

Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer

Patient-derived xenograft (PDX) models and transplantable genetically engineered mouse models faithfully recapitulate human disease and are preferred models for basic and translational breast cancer research. Here, a method is described to orthotopically transplant breast tumor fragments into the mammary fat pad to study tumor biology and evaluate drug...
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Bioluminescent Imaging of Breast Tumor Progression in a Female Mouse Model03:46

Bioluminescent Imaging of Breast Tumor Progression in a Female Mouse Model

Source: Marina Arantes Radicchi1, Victor Carlos Mello1, Victória Paz Machado1, Márcia Cristina Oliveira da Rocha1, Jaqueline Vaz de Oliveira1, Luana Cristina Camargo1,2, Luís Alexandre Muehlmann1, Ricardo Bentes Azevedo1, Sônia Nair Bao1, João Paulo Figueiró Longo11Institute of Biological Sciences, University of Brasília, 2Department of Basic Psychological Processes, Institute of Psychology, University of BrasíliaThis video demonstrates the...
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Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors07:01

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

The aim of this methodology is to identify cancer stem cells (CSC) in cancer cell lines and primary human tumor samples with the sphere-forming protocol, in a robust manner, using functional assays and phenotypic characterization with flow cytometry and Western...
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Terahertz Imaging and Characterization Protocol for Freshly Excised Breast Cancer Tumors08:56

Terahertz Imaging and Characterization Protocol for Freshly Excised Breast Cancer Tumors

Freshly excised human breast cancer tumors are characterized with terahertz spectroscopy and imaging following fresh tissue handling protocols. Tissue positioning is taken into consideration to enable effective characterization while providing analysis in a timely manner for future intraoperative...
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Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer07:13

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Activation of latent mutations with adenovirus-Cre into the mammary ductal system results in a clinically relevant metastatic breast cancer. Incorporation of a YFP promoter allows tracking of distal metastatic tumor cells. This model is useful to study latent metastasis, anti-tumor immunity, and for designing novel immunotherapies to treat breast...
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Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer08:26

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer

We describe a method for imaging response to anti-cancer treatment in vivo and at single cell...
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