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Long non-coding RNA ENST00000434223 inhibits the progression of renal cancer through Wnt/hygro-catenin signaling

N-Q Luo1, D-R Ma, X-C Yang

  • 1Department of Urology, Gansu Provincial Hospital, Lanzhou, China. huangxiande720@sina.com.

European Review for Medical and Pharmacological Sciences
|September 6, 2019
PubMed
Abstract

Insights

Long non-coding RNA ENST00000434223 (LncRNA ENST) inhibits renal cancer progression. This study found LncRNA ENST suppresses tumor viability, metastasis, and invasiveness via the Wnt/β-catenin pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Renal carcinoma is a significant health concern.
  • Long non-coding RNAs (LncRNAs) play crucial roles in various cancers.
  • The specific function of LncRNA ENST00000434223 in renal cancer remains largely unexplored.

Purpose of the Study:

  • To elucidate the functional role of LncRNA ENST00000434223 in renal carcinoma.
  • To investigate the underlying molecular mechanisms involving the Wnt/β-catenin pathway.

Main Methods:

  • Quantitative Real-Time-Polymerase Chain Reaction (qRT-PCR) for expression analysis.
  • Cell proliferation, metastasis, and invasion assays (CCK-8, Transwell).
  • Wnt/β-catenin pathway activation assessment (Luciferase reporter assay, Western blot).

Main Results:

  • LncRNA ENST00000434223 expression is reduced in renal carcinoma, correlating with poor prognosis.
  • Downregulation of LncRNA ENST00000434223 promotes cancer cell viability, metastasis, and invasion.
  • Modulating LncRNA ENST00000434223 affects Wnt/β-catenin pathway signaling proteins.

Conclusions:

  • LncRNA ENST00000434223 acts as a tumor suppressor in renal cancer.
  • Inhibition of renal cancer progression by LncRNA ENST00000434223 is mediated through the Wnt/β-catenin signaling pathway.

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