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MicroRNA-based Regulation of Picornavirus Tropism
Published on: February 6, 2017
MicroRNA-26a regulates cerebral ischemia injury through targeting PTEN
1Department of Neurology, Yantai City Yantai Mountain Hospital, Yantai, China. htopqy@163.com.
Objective:
MicroRNA-26a (miR-26a) exhibits diverse functions in different human disease. However, further research is needed to investigate the potential role of miR-26a in cerebral ischemia injury.
Materials And Methods:
The expressions of miR-26a and PTEN (phosphatase and tensin homolog) were detected via Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) assay. The protein expression of Bcl-2 and Bax was detected by Western blot assay. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to observe the cell viability of SH-SY5Y cells. The relationship between miR-26a and PTEN was confirmed by Dual-Luciferase assay and human SH-SY5Y cells were treated with oxygen-glucose deprivation (OGD)/reperfusion to mimic I/R injury.
Results:
The expression of miR-26a was increased in the OGDR model. Moreover, upregulation of miR-26a promoted cell viability and inhibited OGDR-induced apoptosis. PTEN was confirmed as a direct target gene for miR-26a. Under OGDR conditions, the expression of PTEN was significantly decreased. Moreover, overexpression of PTEN inhibited cell viability, promoted cell apoptosis and deepened the effect of the OGDR model.
Conclusions:
MiR-26a promoted the viability of SH-SY5Y cells and suppressed apoptosis under OGDR conditions by targeting PTEN.
Insights
MicroRNA-26a (miR-26a) protects against cerebral ischemia injury by enhancing cell viability and reducing apoptosis. This neuroprotective effect is mediated through its target gene, PTEN (phosphatase and tensin homolog).
Area of Science:
- Neuroscience
- Molecular Biology
- Biochemistry
Background:
- MicroRNA-26a (miR-26a) has varied roles in human diseases.
- Its specific function in cerebral ischemia injury requires further investigation.
Purpose of the Study:
- To explore the role of miR-26a in cerebral ischemia injury.
- To identify the molecular mechanisms underlying miR-26a's function in this condition.
Main Methods:
- Real-Time quantitative Polymerase Chain Reaction (RT-qPCR) for gene expression.
- Western blot for protein analysis.
- MTT assay for cell viability.
- Dual-Luciferase assay for gene targeting.
- Oxygen-Glucose Deprivation/Reperfusion (OGD/R) model in SH-SY5Y cells.
Main Results:
- miR-26a expression increased in the OGD/R model.
- Upregulated miR-26a enhanced cell viability and inhibited apoptosis.
- PTEN was identified as a direct target of miR-26a.
- PTEN expression decreased under OGD/R conditions.
- PTEN overexpression reduced cell viability and promoted apoptosis.
Conclusions:
- miR-26a promotes SH-SY5Y cell viability and suppresses apoptosis under OGD/R conditions.
- This effect is achieved by targeting PTEN.
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