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Denver Papillae Protocol for Objective Analysis of Fungiform Papillae10:50

Denver Papillae Protocol for Objective Analysis of Fungiform Papillae

Here we present a protocol to measure fungiform papilla density from digital photographs. This method builds prioritization and objective characteristic metrics into the original descriptive work on fungiform papillae by Miller & Reedy...
14.8K
An Experimental Study on Colorado Potato Beetle Hibernation Under Natural Conditions07:44

An Experimental Study on Colorado Potato Beetle Hibernation Under Natural Conditions

Here we present a method for studying Colorado potato beetle hibernation under the natural conditions of the temperate zone as well as a technique for collecting beetles in winter. This method allows to obtain a desired number of overwintering individuals for various analyses at any stage of...
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In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer08:55

In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer

The endothelial glycocalyx/endothelial surface layer is ideally studied using intravital microscopy. Intravital microscopy is technically challenging in a moving organ such as the lung. We demonstrate how simultaneous brightfield and fluorescent microscopy may be used to estimate endothelial surface layer thickness in a freely-moving in vivo mouse...
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Automated Measurement of Microcirculatory Blood Flow Velocity in Pulmonary Metastases of Rats10:56

Automated Measurement of Microcirculatory Blood Flow Velocity in Pulmonary Metastases of Rats

A method is presented to measure microcirculatory blood flow velocity in pulmonary cancer metastases of the pleural surface in rats in an automated fashion, using closed-chest pulmonary intravital microscopy. This model has potential to be used as a widespread tool to perform physiologic research on pulmonary metastases in...
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A Large Animal Model for Pulmonary Hypertension and Right Ventricular Failure: Left Pulmonary Artery Ligation and Progressive Main Pulmonary Artery Banding in Sheep07:02

A Large Animal Model for Pulmonary Hypertension and Right Ventricular Failure: Left Pulmonary Artery Ligation and Progressive Main Pulmonary Artery Banding in Sheep

This manuscript describes the surgical technique and experimental approach to develop severe right ventricular pressure overload to model their adaptive and maladaptive...
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Oleic Acid-Injection in Pigs As a Model for Acute Respiratory Distress Syndrome06:06

Oleic Acid-Injection in Pigs As a Model for Acute Respiratory Distress Syndrome

In this article, we present a protocol to induce acute lung injury in pigs by central-venous injection of oleic acid. This is an established animal model for studying the acute respiratory distress syndrome...
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