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Related Concept Videos

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Intracellular Photothermal Delivery for Suspension Cells Using Sharp Nanoscale Tips in Microwells.

Tianxing Man1, Xiongfeng Zhu1, Yu Ting Chow1

  • 1Department of Mechanical and Aerospace Engineering , University of California, Los Angeles , Los Angeles , California 90095 , United States.

ACS Nano
|September 6, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a novel photothermal delivery method using nanoscale tips to efficiently transfer biomolecules into suspension cells. This technique achieves high efficiency and cell viability, advancing cancer immunotherapy research.

Keywords:
intracellular deliverymicrowellphotothermal deliveryself-aligned cell seedingsuspension cells

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Nanotechnology

Background:

  • Intracellular delivery into suspension cells is crucial for biomedical research, including cancer immunotherapy.
  • Existing delivery platforms face challenges in achieving high efficiency and viability for suspension cells.

Purpose of the Study:

  • To develop a high-efficiency photothermal delivery approach for intracellular delivery into suspension cells.
  • To demonstrate the platform's capability for delivering various biomolecules while maintaining cell viability.

Main Methods:

  • Utilized self-aligned microfabrication to create microwell arrays with nanoscale metallic sharp tips.
  • Employed laser-induced cavitation bubbles for controllable membrane disruption and cargo delivery.
  • Tested delivery efficiency using Calcein green, FITC-dextran, β-lactamase, and a green fluorescence protein expression plasmid in Ramos suspension B cells.

Main Results:

  • Achieved >84% efficiency for 0.6 kDa Calcein green and >45% for 2000 kDa FITC-dextran.
  • Maintained cell viability >96% with a throughput exceeding 100,000 cells per minute.
  • Demonstrated successful delivery and retained activity of β-lactamase and high transfection efficiency (>58%) for GFP plasmid with >89% viability.

Conclusions:

  • The photothermal delivery platform offers a highly efficient and viable method for intracellular delivery into suspension cells.
  • This technology has significant potential for applications in cancer immunotherapy and other cell-based research.
  • The platform's scalability and efficiency address key limitations of current intracellular delivery systems.