Related Experiment Video
Updated: Jan 20, 2026

Extraction of High Molecular Weight DNA from Microbial Mats
Published on: July 7, 2011
Physicochemical properties and bioactivities of original and Se-enriched polysaccharides with different molecular
Li Wang1, Peng-Zhan Zhang1, Jin-Wen Shen2
1Department of Traditional Chinese Medicine, Henan Agricultural University, Zhengzhou 450001, China.
Abstract:
Three polysaccharides (WZP1, WZP2, WZP3) and their Se-enriched products (SeWZP1, SeWZP2 and SeWZP3) were obtained from Pleurotus ostreatus using a simple, rapid method and HNO3-Na2SeO3 method, respectively. The molecular weight distribution profiles of all samples except SeWZP2 showed double peaks. The average molecular weights (Mw) of WZP1-3 were 48.6 kDa, 20.2 kDa and 11.8 kDa, respectively, and of SeWZP1-3 were 19.6 kDa, 37.7 kDa, 14.5 kDa, respectively. The complexity of monosaccharide composition of WZP1-3 was inversely proportional to the ethanol concentration used in the ethanol precipitation process. Additionally, the results of biological activity tests indicated that α-glucosidase inhibitory activity of WZP1-3 was related to the molecular weight and the monosaccharide composition complexity. The selenized modification can improve the α-glucosidase-inhibiting, hydroxyl radical-scavenging activity of P. ostreatus polysaccharides. Therefore, by improving their bioactivities by selenization, the polysaccharides of P. ostreatus could be utilized as a natural health food supplement.
More Related Videos
Related Concept Videos
09:30Extraction of High Molecular Weight DNA from Microbial Mats
11:24Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
06:54Enhanced Extraction of Low-Molecular Weight DNA from Wastewater for Comprehensive Assessment of Antimicrobial Resistance
Factors Affecting Drug Biotransformation: Physicochemical and Chemical Properties of Drugs
The drug's acidity or basicity is essential in...
Polymers: Defining Molecular Weight
The number average molecular weight (Mn) is the summation of the number...
Polymers: Molecular Weight Distribution

