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Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia12:42

Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia

Brain myeloid cells characterization following stroke can be performed by stereology using the optical fractionator method, or by a flow cytometric analysis of brain leukocytes suspensions. Both are useful techniques to perform an accurate phenotypical distinction of the main myeloid cell subsets found in the ischemic...
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Assessment of apical pulse01:17

Assessment of apical pulse

Assessing the Apical Pulse
Assessing the apical pulse is a critical nursing procedure, particularly indicated for:
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In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis09:11

In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis

This protocol describes an apical-out necrotizing enterocolitis (NEC)-in-a-dish model utilizing small intestinal enteroids with reversed polarity, allowing access to the apical surface. We provide an immunofluorescent staining protocol to detect NEC-related epithelial disruption and a method to determine the viability of apical-out enteroids subjected to the NEC-in-a-dish...
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Pseudofracture: An Acute Peripheral Tissue Trauma Model10:08

Pseudofracture: An Acute Peripheral Tissue Trauma Model

Pseudofracture, a reproducible murine model of sterile musculoskeletal trauma, allows for evaluation of late term post-traumatic immune responses. This article describes the procedural execution of the model step by step, including the potential for experimental model combinations to permit study of multiple...
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Focused Assessment with Sonography for Trauma (FAST) Exam: Image Acquisition07:18

Focused Assessment with Sonography for Trauma (FAST) Exam: Image Acquisition

The Focused Assessment with Sonography for Trauma (FAST) exam is a diagnostic point-of-care ultrasound examination used to screen for the presence of free fluid in the pericardium and peritoneum. Indications, techniques, and pitfalls of the procedure are discussed in this article.
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Investigating Intestinal Barrier Breakdown in Living Organoids07:18

Investigating Intestinal Barrier Breakdown in Living Organoids

Here we describe a technique to quantify the barrier integrity of small intestinal organoids. The fact that the method is based on living organoids enables the sequential investigation of different barrier integrity modulating substances or combinations thereof in a time-resolved...
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