Related Concept Videos

Efficient Method for Imaging Murine Lungs that Preserves Spatial Dynamics of Fungal Spores in the Airways10:06

Efficient Method for Imaging Murine Lungs that Preserves Spatial Dynamics of Fungal Spores in the Airways

We present a method for a fungal pathogenesis model that preserves the natural positioning of fungal spores in the lung airways for analysis via fluorescent microscopy.
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A Technique to Quantify Fungal Infection in Zebrafish Larvae02:24

A Technique to Quantify Fungal Infection in Zebrafish Larvae

This video demonstrates a method to quantify fungal infection in zebrafish larvae. Fungal spores are microinjected into the larval hindbrain ventricle, and the larvae are incubated to allow infection development. Infected larvae are harvested at defined intervals, and infection progress is assessed by enumerating colony-forming...
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A Technique to Develop a Parasite Infection in Intestinal Organoids via Microinjection02:33

A Technique to Develop a Parasite Infection in Intestinal Organoids via Microinjection

This video demonstrates a microinjection technique to develop a human intestinal organoid infection model. The infectious sporozoite stage of the parasite Cryptosporidium parvum is microinjected into the organoid lumen. Upon invading the epithelial cells of the organoid, it undergoes asexual and sexual reproduction to complete its life cycle and spreads the...
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Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions09:42

Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions

This protocol describes an Aspergillus infection model in zebrafish larvae. Aspergillus spores are microinjected into the hindbrain of larvae, and chemical treatment is used to induce immunosuppression. Infection progression is monitored via a daily imaging setup to monitor fungal growth and immune responses as well as enumeration of live spores by colony forming unit...
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Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding02:26

Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding

This video demonstrates the establishment of rice plant leaf infection through wounding inoculation with Magnaporthe grisea mycelium. The fungal spore-derived germ tube penetrates the epidermal layer, resulting in tissue infection and lesions on the leaves. This method aids pathotype screening and improves plant resistance understanding for molecular...
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A zWEDGI Technique to Visualize Fungal Pathogen-Infected Zebrafish Larvae02:58

A zWEDGI Technique to Visualize Fungal Pathogen-Infected Zebrafish Larvae

The video demonstrates a zebrafish wounding and entrapment device technique for observing fluorescently labeled fungal pathogen infection in zebrafish larvae. Initial infection attracts macrophages, and as infection persists, neutrophil recruitment progresses at the injection...
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