Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Catalysis02:50

Catalysis

30.1K
The presence of a catalyst affects the rate of a chemical reaction. A catalyst is a substance that can increase the reaction rate without being consumed during the process. A basic comprehension of a catalysts’ role during chemical reactions can be understood from the concept of reaction mechanisms and energy diagrams.
30.1K
Introduction to Catalysis08:23

Introduction to Catalysis

36.1K
Source: Laboratory of Dr. Ryan Richards — Colorado School of Mines
Catalysis is among the most important fields of modern technology and presently accounts for approximately 35% of the gross domestic product (GDP) and sustenance of approximately 33% of the global population through fertilizers produced via the Haber process.1 Catalysts are systems that facilitate chemical reactions by lowering the activation energy and influencing the selectivity. Catalysis will be a central technology in...
36.1K
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

10.5K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
10.5K
Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions12:23

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

8.9K
Effector translocation into host cells via a type III secretion system is a common virulence strategy among gram-negative bacteria. A beta-lactamase effector fusion based assay for quantitative analysis of translocation was applied. In Yersinia infected cells, conversion of a FRET reporter by the beta-lactamase is monitored using laser scanning...
8.9K
Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle15:57

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

12.7K
Long-term cultured interferon-γ enzyme-linked immunospot assay is used as a measure of central memory responses and correlates with protective anti-mycobacterial vaccine responses. With this assay, peripheral blood mononuclear cells are stimulated with mycobacterial antigens and interleukin-2 for 14 days, enabling differentiation and expansion of central memory T...
12.7K
Electroporation of Functional Bacterial Effectors into Mammalian Cells08:39

Electroporation of Functional Bacterial Effectors into Mammalian Cells

10.4K
Electroporation was used to insert purified bacterial virulence effector proteins directly into living eukaryotic cells. Protein localization was monitored by confocal immunofluorescence microscopy. This method allows for studies on trafficking, function, and protein-protein interactions using active exogenous proteins, avoiding the need for heterologous expression in eukaryotic cells.
10.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Escaping the Iron Law of Electrochemical CO<b><sub>2</sub></b> Reduction Using Pd<sub>12</sub>L<sub>24</sub> Cages as Artificial 2nd Coordination Spheres.

Inorganic chemistry·2026
Same author

Cooperative Dinuclear Activation of a Formate Intermediate in the Hydrogenation of CO<sub>2</sub> to Methanol.

Molecules (Basel, Switzerland)·2026
Same author

Catalysis<sup>cubed</sup> Reloaded─Cyclopropanations inside Porphyrin-Based Supramolecular M<sub>8</sub>L<sub>6</sub> Aggregates.

Inorganic chemistry·2026
Same author

Regio- and Enantioselective Alkoxycarbonylation of Unactivated Terminal Alkenes under Palladium-Bromide-Monophosphine Catalysis.

Journal of the American Chemical Society·2026
Same author

Synthesis and characterisation of novel dipyridine and pyridinyl benzoquinoline complexes of zinc and nickel.

Dalton transactions (Cambridge, England : 2003)·2026
Same author

From [NHC─H]<sup>•</sup> to Persistent σ-Complex Radicals: Photoinduced Radical Chemistry of Imidazolium Salts.

Angewandte Chemie (International ed. in English)·2026

Related Experiment Video

Updated: Jan 20, 2026

Catalysis: Homogenious and Heterogeneous Catalysis
02:50

Catalysis: Homogenious and Heterogeneous Catalysis

30.1K

Effector responsive hydroformylation catalysis.

Shao-Tao Bai1, Vivek Sinha1, Alexander M Kluwer2

  • 1Homogeneous Supramolecular and Bio-inspired Catalysis , Van't Hoff Institute for Molecular Sciences (HIMS) , University of Amsterdam (UvA) , Science Park 904 , 1098 XH Amsterdam , The Netherlands .

Chemical Science
|September 7, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a supramolecular rhodium complex that switches between active dimeric and monomeric forms. This switch, controlled by effector molecules, allows tunable catalytic hydroformylation activity and selectivity.

More Related Videos

Catalysis: Homogeneous and Heterogeneous Catalysts
08:23

Catalysis: Homogeneous and Heterogeneous Catalysts

36.1K
Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
12:23

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Published on: October 13, 2015

8.9K

Related Experiment Videos

Last Updated: Jan 20, 2026

Catalysis: Homogenious and Heterogeneous Catalysis
02:50

Catalysis: Homogenious and Heterogeneous Catalysis

30.1K
Catalysis: Homogeneous and Heterogeneous Catalysts
08:23

Catalysis: Homogeneous and Heterogeneous Catalysts

36.1K
Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
12:23

Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions

Published on: October 13, 2015

8.9K

Area of Science:

  • Supramolecular Chemistry
  • Organometallic Chemistry
  • Catalysis

Background:

  • Rhodium complexes are crucial catalysts in hydroformylation.
  • Controlling catalytic activity and selectivity is key for efficient chemical synthesis.
  • Supramolecular systems offer unique platforms for catalyst design and regulation.

Purpose of the Study:

  • To develop a supramolecular rhodium complex capable of forming catalytically active dimeric or monomeric species.
  • To investigate the role of effector molecules in modulating the complex's speciation and catalytic behavior.
  • To create an effector-responsive catalytic system for hydroformylation.

Main Methods:

  • X-ray crystallography to determine structural features.
  • In situ high-pressure (HP) NMR and IR spectroscopy to monitor species under reaction conditions.
  • Molecular modeling to understand effector binding and speciation.
  • Catalytic and kinetic experiments to evaluate performance.

Main Results:

  • The supramolecular rhodium complex forms dimeric species in the absence of effectors.
  • Binding of effector molecules (e.g., acetate) to the integrated receptor induces formation of monomeric Rh-species.
  • The monomeric and dimeric species exhibit distinct catalytic properties.
  • Effector-induced shifts in equilibrium between dimeric and monomeric forms influence hydroformylation selectivity and activity.

Conclusions:

  • A supramolecular catalytic system was designed where effector binding controls the active rhodium species.
  • This system mimics natural protein regulation by responding to external stimuli.
  • The ability to tune hydroformylation via effector-controlled speciation offers a novel approach to catalyst design.